-
Je něco špatně v tomto záznamu ?
Synovial fluid alpha-defensins in Lyme arthritis-a useful marker
P. Melicherčík, M. Mazura, M. Hodík, K. Dundrová, I. Landor, D. Jahoda, R. Horváth, V. Barták, R. Kizek, E. Klapková
Jazyk angličtina Země Česko
Typ dokumentu časopisecké články
Grantová podpora
9777
Ministerstvo Zdravotnictví Ceské Republiky
- MeSH
- alfa-defensiny * metabolismus analýza MeSH
- biologické markery * analýza MeSH
- C-reaktivní protein analýza MeSH
- dospělí MeSH
- infekční artritida mikrobiologie diagnóza imunologie MeSH
- lidé středního věku MeSH
- lidé MeSH
- lymeská nemoc * diagnóza imunologie mikrobiologie MeSH
- senioři MeSH
- synoviální tekutina * chemie imunologie mikrobiologie MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
Lyme arthritis, one of the possible late manifestations of Lyme borreliosis, predominantly affects the supporting joints and in adults most often occurs in the form of monoarthritis of the knee. Early diagnosis is based on clinical findings and serology. PCR detection of Borrelia in synovial fluid has become an integral part of the laboratory testing algorithm. The clinical presentation and inflammatory markers in Lyme arthritis can resemble septic arthritis. Determining the levels of alpha-defensins (human neutrophil peptide (HNP 1-3)) in synovial fluid by liquid chromatography is a highly sensitive method revealing the presence of inflammatory process. Between 2020 and 2022, we examined eleven patients with Lyme arthritis of the knee. We measured levels of HNP 1-3 from synovial fluid by HPLC in patients, and we compared it with the corresponding C-reactive protein (CRP) levels in paired serum samples. In patients diagnosed with Lyme arthritis, HNP 1-3 levels in synovial fluid ranged from 2.5 to 261 mg/L, with a median of 46.5 mg/L. Average serum CRP was 43 mg/L. The results show that elevated HNP 1-3 can be consistent with not only septic arthritis or systemic disease, but also with Lyme arthritis, especially in patients with negative culture and 16S PCR from synovial fluid. Final diagnosis must be verified by examination for anti-Borrelia antibodies from serum and synovial fluid. The aim of this work is to introduce an HPLC method for the determination of alpha-defensins as one of the possible diagnostic markers.
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc24020970
- 003
- CZ-PrNML
- 005
- 20241105105520.0
- 007
- ta
- 008
- 241105s2024 xr f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1007/s12223-024-01173-0 $2 doi
- 035 __
- $a (PubMed)38869776
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xr
- 100 1_
- $a Melicherčík, Pavel $u Department of Orthopedics, Charles University, First Faculty of Medicine and Motol University Hospital, V Úvalu 84, 150 06, Prague 5, Czech Republic
- 245 10
- $a Synovial fluid alpha-defensins in Lyme arthritis-a useful marker / $c P. Melicherčík, M. Mazura, M. Hodík, K. Dundrová, I. Landor, D. Jahoda, R. Horváth, V. Barták, R. Kizek, E. Klapková
- 520 9_
- $a Lyme arthritis, one of the possible late manifestations of Lyme borreliosis, predominantly affects the supporting joints and in adults most often occurs in the form of monoarthritis of the knee. Early diagnosis is based on clinical findings and serology. PCR detection of Borrelia in synovial fluid has become an integral part of the laboratory testing algorithm. The clinical presentation and inflammatory markers in Lyme arthritis can resemble septic arthritis. Determining the levels of alpha-defensins (human neutrophil peptide (HNP 1-3)) in synovial fluid by liquid chromatography is a highly sensitive method revealing the presence of inflammatory process. Between 2020 and 2022, we examined eleven patients with Lyme arthritis of the knee. We measured levels of HNP 1-3 from synovial fluid by HPLC in patients, and we compared it with the corresponding C-reactive protein (CRP) levels in paired serum samples. In patients diagnosed with Lyme arthritis, HNP 1-3 levels in synovial fluid ranged from 2.5 to 261 mg/L, with a median of 46.5 mg/L. Average serum CRP was 43 mg/L. The results show that elevated HNP 1-3 can be consistent with not only septic arthritis or systemic disease, but also with Lyme arthritis, especially in patients with negative culture and 16S PCR from synovial fluid. Final diagnosis must be verified by examination for anti-Borrelia antibodies from serum and synovial fluid. The aim of this work is to introduce an HPLC method for the determination of alpha-defensins as one of the possible diagnostic markers.
- 650 _2
- $a lidé $7 D006801
- 650 12
- $a synoviální tekutina $x chemie $x imunologie $x mikrobiologie $7 D013582
- 650 12
- $a alfa-defensiny $x metabolismus $x analýza $7 D023084
- 650 12
- $a lymeská nemoc $x diagnóza $x imunologie $x mikrobiologie $7 D008193
- 650 12
- $a biologické markery $x analýza $7 D015415
- 650 _2
- $a mužské pohlaví $7 D008297
- 650 _2
- $a ženské pohlaví $7 D005260
- 650 _2
- $a lidé středního věku $7 D008875
- 650 _2
- $a dospělí $7 D000328
- 650 _2
- $a senioři $7 D000368
- 650 _2
- $a C-reaktivní protein $x analýza $7 D002097
- 650 _2
- $a infekční artritida $x mikrobiologie $x diagnóza $x imunologie $7 D001170
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Mazura, Matěj $u Department of Orthopedics, Charles University, First Faculty of Medicine and Motol University Hospital, V Úvalu 84, 150 06, Prague 5, Czech Republic
- 700 1_
- $a Hodík, Martin $u Department of Medical Chemistry and Clinical Biochemistry, Charles University, Second Faculty of Medicine and Motol University Hospital, V Úvalu 84, 150 06, Prague, Czech Republic
- 700 1_
- $a Dundrová, Kamila $u Department of Medical Microbiology, Charles University, Second Faculty of Medicine and Motol University Hospital, V Úvalu 84, 150 06, Prague, Czech Republic
- 700 1_
- $a Landor, Ivan $u Department of Orthopedics, Charles University, First Faculty of Medicine and Motol University Hospital, V Úvalu 84, 150 06, Prague 5, Czech Republic $1 https://orcid.org/0000000266574299 $7 xx0062301
- 700 1_
- $a Jahoda, David $u Department of Orthopedics, Charles University, First Faculty of Medicine and Motol University Hospital, V Úvalu 84, 150 06, Prague 5, Czech Republic $1 https://orcid.org/0000000284590778 $7 xx0014721
- 700 1_
- $a Horváth, Rudolf $u Department of Rheumatology of Children and Adults, Charles University, Second Faculty of Medicine and Motol University Hospital, V Úvalu 84, 150 06, Prague, Czech Republic
- 700 1_
- $a Barták, Vladislav $u Department of Orthopedics, Charles University, First Faculty of Medicine and Motol University Hospital, V Úvalu 84, 150 06, Prague 5, Czech Republic $1 https://orcid.org/0000000290711943 $7 xx0093686
- 700 1_
- $a Kizek, René $u Department of Medical Chemistry and Clinical Biochemistry, Charles University, Second Faculty of Medicine and Motol University Hospital, V Úvalu 84, 150 06, Prague, Czech Republic
- 700 1_
- $a Klapková, Eva $u Department of Medical Chemistry and Clinical Biochemistry, Charles University, Second Faculty of Medicine and Motol University Hospital, V Úvalu 84, 150 06, Prague, Czech Republic. eva.klapkova@email.cz $1 https://orcid.org/0000000299035662
- 773 0_
- $w MED00011005 $t Folia microbiologica $x 1874-9356 $g Roč. 69, č. 6 (2024), s. 1355-1362
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/38869776 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y - $z 0
- 990 __
- $a 20241105 $b ABA008
- 991 __
- $a 20241105105517 $b ABA008
- 999 __
- $a ok $b bmc $g 2209287 $s 1232960
- BAS __
- $a 3
- BAS __
- $a PreBMC-MEDLINE
- BMC __
- $a 2024 $b 69 $c 6 $d 1355-1362 $e 20240613 $i 1874-9356 $m Folia microbiologica $n Folia Microbiol (Praha) $x MED00011005
- GRA __
- $a 9777 $p Ministerstvo Zdravotnictví Ceské Republiky
- LZP __
- $a Pubmed-20241105
