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Cationic fluorinated micelles for cell labeling and 19F-MR imaging
N. Jirát-Ziółkowska, VM. Panakkal, K. Jiráková, D. Havlíček, O. Sedláček, D. Jirák
Language English Country England, Great Britain
Document type Journal Article
Grant support
IN00023001
Ministerstvo Zdravotnictví Ceské Republiky
LM2018124
Ministerstvo Školství, Mládeže a Tělovýchovy
LX22NPO5104
National Institute for Research of Metabolic and Cardiovascular Diseases
CZ.02.1.01/0.0/0.0/16_013/0001821
European Structural and Investments Funds in the frame of the Research Development and Education
NLK
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- MeSH
- Staining and Labeling methods MeSH
- Phantoms, Imaging MeSH
- Fluorine chemistry MeSH
- Halogenation MeSH
- Cations * chemistry MeSH
- Contrast Media chemistry MeSH
- Humans MeSH
- Magnetic Resonance Imaging methods MeSH
- Micelles * MeSH
- Mice MeSH
- Cell Survival * drug effects MeSH
- Fluorine-19 Magnetic Resonance Imaging methods MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
Magnetic resonance imaging (MRI) relies on appropriate contrast agents, especially for visualizing transplanted cells within host tissue. In recent years, compounds containing fluorine-19 have gained significant attention as MRI probe, particularly in dual 1H/19F-MR imaging. However, various factors affecting probe sensitivity, such as fluorine content and the equivalency of fluorine atoms, must be considered. In this study, we synthesized fluorinated micelles with adjustable surface positive charge density and investigated their physicochemical properties and MRI efficacy in phantoms and labeled cells. While the micelles exhibited clear signals in 19F-MR spectra and imaging, the concentrations required for MRI visualization of labeled cells were relatively high, adversely affecting cell viability. Despite their favourable physicochemical properties, achieving higher labeling rates without compromising cell viability during labeling remains a challenge for potential in vivo applications.
3rd Faculty of Medicine Charles University Ruská 87 Prague 100 00 Czech Republic
Institute for Clinical and Experimental Medicine Vídeňská 1958 9 Prague 140 21 Czech Republic
References provided by Crossref.org
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