Význam hybridizacných metód v mikrobiologickej diagnostike: DNA sondy a PCR
[Significance of hybridization methods in microbiological diagnosis: DNA probes and PCR]
Language Slovak Country Czech Republic Media print
Document type English Abstract, Journal Article, Review
PubMed
1388106
- MeSH
- Bacterial Infections diagnosis MeSH
- DNA Probes * MeSH
- Microbiological Techniques * MeSH
- Polymerase Chain Reaction * MeSH
- Virus Diseases diagnosis MeSH
- Publication type
- English Abstract MeSH
- Journal Article MeSH
- Review MeSH
- Names of Substances
- DNA Probes * MeSH
The authors give an account of hybridization methods, in particular DNA probes and the polymerase chain reaction (PCR) and their application in microbiological diagnosis. They deal with the principle of the two methods and their applications in the diagnosis not only of genera and species but also different factors of pathogenicity and virulence. The authors mention the advantages and disadvantages of radioactively and non-radioactively labelled probes such as PCR. They draw attention to the perspective application of these methods in research and diagnostic laboratories.
