Phenylmethylsulfonyl fluoride protects L-lysine transport in Schizosaccharomyces pombe against inactivation by ammonium ions
Language English Country Netherlands Media print
Document type Comparative Study, Journal Article
PubMed
2110481
DOI
10.1016/0005-2736(90)90129-c
PII: 0005-2736(90)90129-C
Knihovny.cz E-resources
- MeSH
- Ammonia pharmacology MeSH
- Biological Transport drug effects MeSH
- Time Factors MeSH
- Phenylmethylsulfonyl Fluoride pharmacology MeSH
- Lysine metabolism MeSH
- Mutation MeSH
- Saccharomyces cerevisiae drug effects genetics metabolism MeSH
- Saccharomycetales metabolism MeSH
- Schizosaccharomyces drug effects metabolism MeSH
- Sulfones pharmacology MeSH
- Publication type
- Journal Article MeSH
- Comparative Study MeSH
- Names of Substances
- Ammonia MeSH
- Phenylmethylsulfonyl Fluoride MeSH
- Lysine MeSH
- Sulfones MeSH
Ammonium ions inactivate the basic amino acid transport system in Schizosaccharomyces pombe in an irreversible manner. The inactivation is accompanied by a 4-fold decrease of KT of L-lysine transport, leaving its Jmax unchanged; phenylmethylsulfonyl fluoride protects the system against inactivation. In contrast, two basic amino acid transport systems in a gap1 mutant of Saccharomyces cerevisiae are influenced by NH4+ ions in such a way that only the Jmax decreases while the KT of L-lysine transport is unchanged. Phenylmethylsulfonyl fluoride does not act here as a protective agent.
References provided by Crossref.org
Thialysine-resistant mutants and uptake of lysine in Schizosaccharomyces pombe
