A simple novel procedure for preparation of herpes simplex virus subunit vaccine
Language English Country Switzerland Media print
Document type Journal Article
PubMed
2903636
Knihovny.cz E-resources
- MeSH
- Chemical Precipitation MeSH
- DNA, Viral isolation & purification MeSH
- Glycoproteins isolation & purification MeSH
- Virus Cultivation MeSH
- Cells, Cultured MeSH
- Guinea Pigs MeSH
- Mice MeSH
- Octoxynol MeSH
- Polyethylene Glycols MeSH
- Antibodies, Viral biosynthesis MeSH
- Simplexvirus classification immunology MeSH
- Ammonium Sulfate MeSH
- Viral Proteins isolation & purification MeSH
- Viral Vaccines isolation & purification MeSH
- Animals MeSH
- Check Tag
- Guinea Pigs MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- DNA, Viral MeSH
- Glycoproteins MeSH
- Nonidet P-40 MeSH Browser
- Octoxynol MeSH
- Polyethylene Glycols MeSH
- Antibodies, Viral MeSH
- Ammonium Sulfate MeSH
- Viral Proteins MeSH
- Viral Vaccines MeSH
A simple procedure for preparation of herpes simplex virus type 1 (HSV-1) subunit vaccine is described. The method is based on treatment of virus-infected cells with a nonionic detergent, removal of cell nuclei by low speed centrifugation, separation of viral nucleocapsids by high speed centrifugation through a sucrose cushion and, finally, precipitation of viral and cellular glycoproteins and proteins with ammonium sulphate (AS). Unlike to acetone precipitation, affinity chromatography on lectins or hydroxylapatite chromatography, AS precipitation repeatedly yielded the best and most reliable results as judged by relative protein content, antigenicity and immunogenicity of the final vaccine product.
Developments in herpes simplex virus vaccines: old problems and new challenges
