• This record comes from PubMed

Kinetic studies of killer toxin K1 binding to yeast cells indicate two receptor populations

. 1994 ; 162 (3) : 211-4.

Language English Country Germany Media print

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/pmid07979876

A recently described new method for determination of killer toxin activity was used for kinetic measurements of K1 toxin binding. The cells of the killer sensitive strain Saccharomyces cerevisiae S6 were shown to carry two classes of toxin binding sites differing widely in their half-saturation constants and maximum binding rates. The low-affinity and high-velocity binding component (KT1 = 2.6 x 10(9) L.U./ml, Vmax1 = 0.19 s-1) probably reflects diffusion-limited binding to cell wall receptors; the high-affinity and low-velocity component (KT2 = 3.2 x 10(7) L.U./ml, Vmax2 = 0.03 s-1) presumably indicates the binding of the toxin to plasma membrane receptors. Adsorption of most of the killer toxin K1 to the surface of sensitive cells occurred within 1 min and was virtually complete within 5 min. The amount of toxin that saturated practically all cell receptors was about 600 lethal units (L.U.) per cell of S. cerevisiae S6.

See more in PubMed

Adv Microb Physiol. 1981;22:93-122 PubMed

Proc Natl Acad Sci U S A. 1990 Aug;87(16):6228-32 PubMed

Biochim Biophys Acta. 1958 Feb;27(2):267-76 PubMed

Experientia. 1990 Feb 15;46(2):193-200 PubMed

Yeast. 1993 Nov;9(11):1207-11 PubMed

Appl Environ Microbiol. 1989 Aug;55(8):2105-2107 PubMed

Agric Biol Chem. 1990;54(2):505-9 PubMed

J Bacteriol. 1977 Feb;129(2):668-77 PubMed

Crit Rev Microbiol. 1991;18(1):47-87 PubMed

Cell. 1984 Mar;36(3):741-51 PubMed

Antonie Van Leeuwenhoek. 1978;44(1):59-77 PubMed

J Bacteriol. 1983 Apr;154(1):161-9 PubMed

J Bacteriol. 1979 Dec;140(3):888-92 PubMed

Virology. 1992 Mar;187(1):333-7 PubMed

Find record

Citation metrics

Archiving options