The effect of purine phosphonomethoxyalkyl derivatives on DNA synthesis in CHO Chinese hamster cells
Jazyk angličtina Země Slovensko Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
8202198
Knihovny.cz E-zdroje
- MeSH
- CHO buňky MeSH
- DNA biosyntéza MeSH
- elongace translace peptidového řetězce účinky léků MeSH
- křečci praví MeSH
- organofosforové sloučeniny farmakologie MeSH
- poškození DNA MeSH
- puriny farmakologie MeSH
- zvířata MeSH
- Check Tag
- křečci praví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- DNA MeSH
- organofosforové sloučeniny MeSH
- puriny MeSH
The inhibition of incorporation of 3H-thymidine and the changes of the rate of nascent DNA chain elongation were investigated in CHO Chinese hamster cells treated with (S)-(3-hydroxy-2-phosphonomethoxypropyl) (HPMP) and N-(2-phosphonomethoxyethyl) (PME) derivatives of adenine (A), guanine (G) and 2,6-diaminopurine (DAP). No direct correlation was observed in PME and HPMP derivatives between cytotoxicity, inhibition of 3H-thymidine incorporation and inhibition of nascent DNA chain elongation. The highest cytotoxicity and inhibition of DNA synthesis were caused by PMEG. The limited extent of inhibition of DNA elongation was encountered in the case of HPMPG and HPMPA. With PMEA, weak inhibition of elongation of DNA was observed only after a prolonged exposure (6 h). None of the investigated drugs induced DNA breaks.
