Two stable rough mutants of Salmonella spp. were studied as live peroral vaccines. The SF1591 mutant of S. typhimurium (Ra chemotype) protected germ-free piglets against subsequent infection with virulent smooth S. typhimurium LT2, whereas a deep-rough mutant of S. minnesota mR595 (Re chemotype) did not. We investigated cytokine and leukocyte profiles in the ilea of gnotobiotic piglets colonized for 1 week either with rough mutants alone or with rough mutants followed by S. typhimurium LT2. The ileal mucosae of piglets associated with strain SF1591 alone were not inflamed. Villi contained activated macrophages, and enterocytes expressed transforming growth factor beta (TGF-beta). Subsequent infection of piglets with S. typhimurium LT2 resulted in immigration of alphabeta T cells and immunoglobulin A (IgA) response. In contrast, the ileal mucosae of piglets associated with strain mR595 alone expressed heat shock proteins and inflammatory cytokines but not TGF-beta. Acellular villi contained numerous gammadelta T cells but no alphabeta T cells. After subsequent challenge with the LT2 strain, most piglets died of sepsis. Intestinal mucosae contained IgG but no IgA. These findings suggest the importance of cytokine signals in the regulation of intestinal responses against Salmonella infection.

Division of Immunology and Gnotobiology Institute of Microbiology Academy of Sciences of the Czech Republic Prague

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Infect Immun. 1991 Dec;59(12):4491-6 PubMed

Folia Microbiol (Praha). 1989;34(2):157-64 PubMed

Microb Pathog. 1991 Nov;11(5):347-56 PubMed

Nature. 1992 Jun 18;357(6379):588-9 PubMed

Clin Immunol Immunopathol. 1992 Oct;65(1):1-9 PubMed

Folia Biol (Praha). 1992;38(3-4):202-15 PubMed

Immunology. 1992 Oct;77(2):219-27 PubMed

Cell Immunol. 1993 Oct 15;151(2):336-44 PubMed

Infect Immun. 1993 Nov;61(11):4569-74 PubMed

Infect Agents Dis. 1993 Apr;2(2):55-73 PubMed

Vet Immunol Immunopathol. 1994 May;41(1-2):31-40 PubMed

Infect Immun. 1995 Mar;63(3):1134-7 PubMed

J Exp Med. 1996 Jul 1;184(1):271-6 PubMed

Immunol Today. 1996 Aug;17(8):352-4 PubMed

Immunol Today. 1996 Feb;17(2):57-9 PubMed

Infect Immun. 1997 Dec;65(12):5238-43 PubMed

J Interferon Res. 1985 Winter;5(1):45-53 PubMed

Immunology. 1988 Jul;64(3):539-44 PubMed

J Clin Immunol. 1992 Mar;12(2):61-74 PubMed

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