Leucine metabolism in TNF-alpha- and endotoxin-treated rats: contribution of hepatic tissue
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Amino Acids blood metabolism MeSH
- Models, Biological MeSH
- Endotoxins pharmacology MeSH
- Liver drug effects metabolism MeSH
- Keto Acids pharmacokinetics MeSH
- Rats MeSH
- Leucine metabolism MeSH
- Humans MeSH
- Lipopolysaccharides pharmacology MeSH
- Mice MeSH
- Rats, Wistar MeSH
- Radioisotope Dilution Technique MeSH
- Carbon Radioisotopes MeSH
- Recombinant Proteins pharmacokinetics pharmacology MeSH
- Salmonella enteritidis MeSH
- Body Weight MeSH
- Tumor Necrosis Factor-alpha pharmacokinetics pharmacology MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Humans MeSH
- Male MeSH
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- alpha-ketoisocaproic acid MeSH Browser
- Amino Acids MeSH
- Endotoxins MeSH
- Keto Acids MeSH
- Leucine MeSH
- Lipopolysaccharides MeSH
- Carbon Radioisotopes MeSH
- Recombinant Proteins MeSH
- Tumor Necrosis Factor-alpha MeSH
The effects of tumor necrosis factor-alpha (TNF-alpha; cachectin) and lipopolysaccharide of Salmonella enteritidis (LPS; endotoxin) on leucine metabolism in rats were evaluated in the whole body using intravenous infusion of L-[1-14C]leucine and in isolated perfused liver (IPL) using the single-pass perfusion technique with alpha-keto[1-14C]isocaproate as a tracer for measurement of ketoisocaproic acid (KIC) oxidation, and the recirculation technique for measurement of hepatic amino acid exchanges. The data obtained in TNF-alpha and LPS groups were compared with those obtained in controls. Both TNF-alpha and LPS treatment induced an increase of whole body leucine turnover, oxidation, and clearance. As the result of a higher increase of leucine oxidation than of incorporation into the pool of body proteins, the fractional oxidation of leucine was increased. The fractional rate of protein synthesis increased significantly in the spleen (both in TNF-alpha and LPS rats), in blood plasma, liver, colon, kidneys, gastrocnemius muscle (in LPS rats), and in lungs (TNF-alpha-treated rats), whereas it decreased in the jejunum (LPS rats). In IPL of TNF-alpha- and LPS-treated rats a decrease of KIC oxidation and higher uptake of branched-chain amino acids (BCAA; valine, leucine, and isoleucine) were observed when compared with control animals. We hypothesize that the negative consequences of increased whole body proteolysis and of increased oxidation of BCAA induced by TNF-alpha and/or LPS are reduced by decreased activity of hepatic branched-chain ketoacid dehydrogenase that can help resupply BCAA to the body.
References provided by Crossref.org
The dose-dependent effects of endotoxin on protein metabolism in two types of rat skeletal muscle
Effects of proteasome inhibitors MG132, ZL3VS and AdaAhx3L3VS on protein metabolism in septic rats
Protein metabolism in guanethidine-treated rats
Acute effects of acidosis on protein and amino acid metabolism in perfused rat liver
