Molecular variability of Czech plum pox virus isolates
Language English Country Switzerland Media print
Document type Journal Article
PubMed
10073232
Knihovny.cz E-resources
- MeSH
- Enzyme-Linked Immunosorbent Assay methods MeSH
- Antibodies, Monoclonal MeSH
- Plant Diseases virology MeSH
- Reverse Transcriptase Polymerase Chain Reaction methods MeSH
- Polymorphism, Restriction Fragment Length MeSH
- Serotyping MeSH
- Plum Pox Virus classification genetics isolation & purification MeSH
- Blotting, Western MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- Czech Republic MeSH
- Names of Substances
- Antibodies, Monoclonal MeSH
Seventy-two Czech plum pox virus (PPV) isolates from different hosts were tested by double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). In addition, the coat protein mobility and the RsaI restriction fragment length polymorphism (RFLP) pattern of reverse transcription-polymerase chain reaction-(RT-PCR) amplified coat protein (CP) gene fragment of the isolates were analysed. Both PPV-D and PPV-M serotypes were found in the Czech Republic. The results obtained by these different methods were in accord with exception of a few cases probably caused by mutation or recombination.
