Involvement of Nha1 antiporter in regulation of intracellular pH in Saccharomyces cerevisiae
Language English Country Great Britain, England Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
10077841
DOI
10.1111/j.1574-6968.1999.tb13428.x
PII: S0378-1097(98)00597-7
Knihovny.cz E-resources
- MeSH
- Biological Transport physiology MeSH
- Cell Membrane metabolism MeSH
- Potassium Chloride pharmacology MeSH
- Sodium Chloride pharmacology MeSH
- Fungal Proteins metabolism MeSH
- Glucose pharmacology MeSH
- Hydrogen-Ion Concentration * MeSH
- Membrane Proteins metabolism MeSH
- Sodium-Hydrogen Exchangers metabolism MeSH
- Cation Transport Proteins * MeSH
- Protons MeSH
- Saccharomyces cerevisiae Proteins * MeSH
- Saccharomyces cerevisiae drug effects metabolism MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Potassium Chloride MeSH
- Sodium Chloride MeSH
- Fungal Proteins MeSH
- Glucose MeSH
- Membrane Proteins MeSH
- Sodium-Hydrogen Exchangers MeSH
- NHA1 protein, S cerevisiae MeSH Browser
- Cation Transport Proteins * MeSH
- Protons MeSH
- Saccharomyces cerevisiae Proteins * MeSH
The Nha1 antiporter is involved in regulation of intracellular pH in Saccharomyces cerevisiae. We report that deletion of the NHA1 gene resulted in an increase of cytoplasmic pH in cells suspended in water or acidic buffers. Addition of KCl or NaCl to exponentially growing cells lowered the internal pH but the difference between cells with or without NHA1 was maintained. Addition of KCl to starved cells resulted in much higher alkalinization of cytoplasmic pH in a strain lacking Nha1p compared to the wild-type or Nha1p-overexpressing strains. The H+/K+(Na+) exchange mechanism of Nha1p was confirmed in reconstituted plasma membrane vesicles.
References provided by Crossref.org
The Toxic Effects of Ppz1 Overexpression Involve Nha1-Mediated Deregulation of K+ and H+ Homeostasis
Functional comparison of plasma-membrane Na+/H+ antiporters from two pathogenic Candida species
Exploration of yeast alkali metal cation/H+ antiporters: sequence and structure comparison
