Nuclear organization studied with the help of a hypotonic shift: its use permits hydrophilic molecules to enter into living cells
Jazyk angličtina Země Rakousko Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
10525969
DOI
10.1007/s004120050384
PII: 91080325.412
Knihovny.cz E-zdroje
- MeSH
- amanitiny metabolismus MeSH
- barvicí látky metabolismus MeSH
- buněčné jádro metabolismus MeSH
- chemické jevy MeSH
- difuze MeSH
- Drosophila melanogaster metabolismus MeSH
- epitelové buňky účinky léků metabolismus MeSH
- fibroblasty účinky léků metabolismus MeSH
- fyzikální chemie MeSH
- HeLa buňky účinky léků metabolismus MeSH
- hypotonické roztoky farmakologie MeSH
- imunoelektronová mikroskopie MeSH
- imunoglobulin G metabolismus MeSH
- játra metabolismus MeSH
- krysa rodu Rattus MeSH
- kultivované buňky MeSH
- léčivé přípravky metabolismus MeSH
- ledviny MeSH
- lidé MeSH
- mikroinjekce MeSH
- molekulová hmotnost MeSH
- nukleotidy metabolismus MeSH
- orgánové kultury - kultivační techniky MeSH
- permeabilita buněčné membrány účinky léků MeSH
- psi MeSH
- stanovení celkové genové exprese MeSH
- uridintrifosfát analogy a deriváty metabolismus MeSH
- viabilita buněk MeSH
- Xenopus laevis MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- lidé MeSH
- mužské pohlaví MeSH
- psi MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- 5-bromouridine triphosphate MeSH Prohlížeč
- amanitiny MeSH
- barvicí látky MeSH
- hypotonické roztoky MeSH
- imunoglobulin G MeSH
- léčivé přípravky MeSH
- nukleotidy MeSH
- uridintrifosfát MeSH
A new procedure for introduction of hydrophilic molecules into living cells based on efficient uptake of these molecules into the cells during hypotonic treatment is presented and its use is demonstrated by a variety of applications. Experiments with cultured vertebrate and Drosophila cells and various animal tissues demonstrated that the increase in cell membrane permeability under hypotonic conditions is a general phenomenon in all animal cells tested. The efficiency of the method depends on the composition and temperature of the hypotonic buffer, the duration of the hypotonic treatment and the molecular weight of the molecules introduced into living cells. The versatility of this approach is demonstrated with various types of molecules such as modified nucleotides, nucleotides with conjugated fluorochrome, peptides, phosphatase substrates and fluorescent dyes. The method opens new possibilities for the direct investigation of a variety of biological problems as documented here with data on the functional organization of the cell nucleus.
Citace poskytuje Crossref.org
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