Drug-induced hypomethylation of a posttranscriptionally silenced transgene locus of tobacco leads to partial release of silencing
Language English Country Great Britain, England Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
10664454
DOI
10.1016/s0014-5793(00)01077-2
PII: S0014-5793(00)01077-2
Knihovny.cz E-resources
- MeSH
- 3' Untranslated Regions genetics MeSH
- Adenine analogs & derivatives pharmacology MeSH
- Azacitidine pharmacology MeSH
- Cytosine metabolism MeSH
- DNA, Bacterial genetics MeSH
- Transcription, Genetic drug effects genetics MeSH
- Plants, Genetically Modified MeSH
- Plants, Toxic * MeSH
- Kanamycin Kinase genetics metabolism MeSH
- Cells, Cultured MeSH
- DNA Methylation drug effects MeSH
- Open Reading Frames genetics MeSH
- Gene Expression Regulation, Plant drug effects MeSH
- Genes, Reporter genetics MeSH
- Nicotiana cytology drug effects genetics metabolism MeSH
- Transgenes genetics MeSH
- Gene Silencing drug effects MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 3' Untranslated Regions MeSH
- 9-(2,3-dihydroxypropyl)adenine MeSH Browser
- Adenine MeSH
- Azacitidine MeSH
- Cytosine MeSH
- DNA, Bacterial MeSH
- Kanamycin Kinase MeSH
- T-DNA MeSH Browser
The effect of DNA methylation upon posttranscriptional gene silencing (PTGS) has been investigated in transgenic tobacco lines showing PTGS and methylation of the neomycin phosphotransferase II (nptII) reporter genes. Application of the hypomethylation drugs dihydroxypropyladenine or 5-azacytidine resulted in approximately 30% reduced methylation of cytosines located in a non-symmetrical context in the 3' untranslated region of the nptII transgenes. The hypomethylation was accompanied by up to 12-fold increase in NPTII protein levels, suggesting that methylation of non-symmetrical motifs may account for an increased degree of PTGS. Models for the possible role of DNA methylation in PTGS are discussed.
References provided by Crossref.org
Compromised telomere maintenance in hypomethylated Arabidopsis thaliana plants
Paramutation of tobacco transgenes by small RNA-mediated transcriptional gene silencing
