The fission yeast ortholog of the coregulator SKIP interacts with the small subunit of U2AF
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
11414703
DOI
10.1006/bbrc.2001.5108
PII: S0006-291X(01)95108-3
Knihovny.cz E-zdroje
- MeSH
- aktivace transkripce MeSH
- buněčný cyklus MeSH
- jaderné proteiny metabolismus MeSH
- molekulární sekvence - údaje MeSH
- ribonukleoproteiny metabolismus MeSH
- Saccharomyces cerevisiae metabolismus MeSH
- Schizosaccharomyces metabolismus MeSH
- sestřihový faktor U2AF MeSH
- techniky dvojhybridového systému MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- jaderné proteiny MeSH
- ribonukleoproteiny MeSH
- sestřihový faktor U2AF MeSH
- U2AF2 protein, human MeSH Prohlížeč
The mode of action of transcriptional coregulators may involve the recruitment of spliceosome components. Using the two-hybrid screen, we examined the interaction partners of spSNW1, the S. pombe ortholog of the human coregulator SNW1/SKIP/NCoA-62, and found it to interact with the small subunit of the splicing factor U2AF (spU2AF23). The interaction involves the C-terminal parts of spU2AF23 and spSNW1. Tagged variants of both proteins were expressed in S. pombe and the interaction was proved by coprecipitation in nuclear extracts. This interaction would explain the finding of SKIP in nuclear speckles (Mintz, P. J., et al., EMBO J. 18, 4308-4320, 1999) and in reconstituted spliceosomes (Neubauer, G., et al., Nat. Genet. 20, 46-50, 1998). We deleted the spSNW1 gene in the diploid strain and demonstrated that spSNW1 is an essential gene in S. pombe.
Citace poskytuje Crossref.org
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GENBANK
AL032824
