Auxin efflux carrier activity and auxin accumulation regulate cell division and polarity in tobacco cells
Jazyk angličtina Země Německo Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- biologický transport účinky léků MeSH
- buněčné dělení účinky léků fyziologie MeSH
- ftalimidy metabolismus farmakologie MeSH
- kultivované buňky MeSH
- kyselina 2,4-dichlorfenoxyoctová metabolismus farmakologie MeSH
- kyseliny indoloctové metabolismus MeSH
- kyseliny naftalenoctové metabolismus farmakologie MeSH
- regulátory růstu rostlin metabolismus farmakologie MeSH
- tabák cytologie účinky léků metabolismus MeSH
- transportní proteiny metabolismus MeSH
- tritium MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- 1-naphthaleneacetic acid MeSH Prohlížeč
- alpha-naphthylphthalamic acid MeSH Prohlížeč
- ftalimidy MeSH
- kyselina 2,4-dichlorfenoxyoctová MeSH
- kyseliny indoloctové MeSH
- kyseliny naftalenoctové MeSH
- regulátory růstu rostlin MeSH
- transportní proteiny MeSH
- tritium MeSH
Division and growth of most types of in vitro-cultured plant cells require an external source of auxin. In such cultures, the ratio of external to internal auxin concentration is crucial for the regulation of the phases of the standard growth cycle. In this report the internal concentration of auxin in suspension-cultured cells of Nicotiana tabacum L., strain VBI-0, was manipulated either (i) by increasing 10-fold the normal concentration of 1-naphthaleneacetic acid (NAA) and 2,4-dichlorophenoxyacetic acid in the external medium; or (ii) by addition 1-N-naphthylphthalamic acid (NPA; an inhibitor of auxin efflux and of auxin efflux carrier traffic). Both treatments delayed the onset of cell division for 6-7 days without loss of cell viability. In both cases, cell division activity subsequently resumed coincident with a reduction in the ability of cells to accumulate [(3)H]NAA from an external medium. Following renewed cell division, a significant proportion of the NPA-treated cells but not those grown at high auxin concentration, exhibited changes in the orientation of new cell divisions and loss of polarity. We conclude that cell division, but not cell elongation, is prevented when the internal auxin concentration rises above a critical threshold value and that the directed traffic of auxin efflux carriers to the plasma membrane may regulate the orientation of cell divisions.
Citace poskytuje Crossref.org
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