Auxin efflux carrier activity and auxin accumulation regulate cell division and polarity in tobacco cells
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Biological Transport drug effects MeSH
- Cell Division drug effects physiology MeSH
- Phthalimides metabolism pharmacology MeSH
- Cells, Cultured MeSH
- 2,4-Dichlorophenoxyacetic Acid metabolism pharmacology MeSH
- Indoleacetic Acids metabolism MeSH
- Naphthaleneacetic Acids metabolism pharmacology MeSH
- Plant Growth Regulators metabolism pharmacology MeSH
- Nicotiana cytology drug effects metabolism MeSH
- Carrier Proteins metabolism MeSH
- Tritium MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 1-naphthaleneacetic acid MeSH Browser
- alpha-naphthylphthalamic acid MeSH Browser
- Phthalimides MeSH
- 2,4-Dichlorophenoxyacetic Acid MeSH
- Indoleacetic Acids MeSH
- Naphthaleneacetic Acids MeSH
- Plant Growth Regulators MeSH
- Carrier Proteins MeSH
- Tritium MeSH
Division and growth of most types of in vitro-cultured plant cells require an external source of auxin. In such cultures, the ratio of external to internal auxin concentration is crucial for the regulation of the phases of the standard growth cycle. In this report the internal concentration of auxin in suspension-cultured cells of Nicotiana tabacum L., strain VBI-0, was manipulated either (i) by increasing 10-fold the normal concentration of 1-naphthaleneacetic acid (NAA) and 2,4-dichlorophenoxyacetic acid in the external medium; or (ii) by addition 1-N-naphthylphthalamic acid (NPA; an inhibitor of auxin efflux and of auxin efflux carrier traffic). Both treatments delayed the onset of cell division for 6-7 days without loss of cell viability. In both cases, cell division activity subsequently resumed coincident with a reduction in the ability of cells to accumulate [(3)H]NAA from an external medium. Following renewed cell division, a significant proportion of the NPA-treated cells but not those grown at high auxin concentration, exhibited changes in the orientation of new cell divisions and loss of polarity. We conclude that cell division, but not cell elongation, is prevented when the internal auxin concentration rises above a critical threshold value and that the directed traffic of auxin efflux carriers to the plasma membrane may regulate the orientation of cell divisions.
References provided by Crossref.org
Salicylic Acid Targets Protein Phosphatase 2A to Attenuate Growth in Plants
What Has Been Seen Cannot Be Unseen-Detecting Auxin In Vivo
Auxin flow-mediated competition between axillary buds to restore apical dominance
Auxin transport at cellular level: new insights supported by mathematical modelling
Auxin influx inhibitors 1-NOA, 2-NOA, and CHPAA interfere with membrane dynamics in tobacco cells
Do phytotropins inhibit auxin efflux by impairing vesicle traffic?
