Differential effect of Bacillus firmus on immune response and enterocyte brush-border enzyme levels in BALB/c and B10.BR mice
Language English Country United States Media print
Document type Journal Article
PubMed
12630333
DOI
10.1007/bf02818685
Knihovny.cz E-resources
- MeSH
- Alkaline Phosphatase metabolism MeSH
- Bacillus immunology MeSH
- beta-Galactosidase metabolism MeSH
- Dipeptidyl Peptidase 4 metabolism MeSH
- Enterocytes enzymology microbiology MeSH
- Genetic Predisposition to Disease MeSH
- Glucan 1,4-alpha-Glucosidase metabolism MeSH
- Germ-Free Life MeSH
- Immunoglobulin A biosynthesis blood MeSH
- Immunohistochemistry MeSH
- Lactase MeSH
- Microvilli enzymology MeSH
- Mice, Inbred BALB C MeSH
- Mice MeSH
- Sucrase metabolism MeSH
- Intestines enzymology immunology microbiology MeSH
- Intestinal Mucosa enzymology immunology microbiology MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Names of Substances
- Alkaline Phosphatase MeSH
- beta-Galactosidase MeSH
- Dipeptidyl Peptidase 4 MeSH
- Glucan 1,4-alpha-Glucosidase MeSH
- Immunoglobulin A MeSH
- Lactase MeSH
- Sucrase MeSH
A nonpathogenic bacterium of external environment possessing remarkable immunomodulatory activity, Bacillus firmus (BF) inactivated with formaldehyde, was given intragastrically to two genetically different mouse strains BALB/c (H-2d) and B10.BR/SnPh (B10.BR, H-2k) reared in conventional (CV) and B10.BR strain also in germ-free (GF) conditions. Repeated intragastric administration of BF (500 micrograms every other day over two weeks, starting at the age of 3 months) significantly enhanced intestinal IgA levels in CV BALB/c mice but did not affect intestinal IgA in CV B10.BR mice. In GF B10.BR mice, IgG levels in sera and intestinal washings increased after BF administration compared to CV B10.BR mice. In CV BALB/c mice, specific activity of enterocyte brush-border enzymes (lactase, gamma-glutamyltransferase, alkaline phosphatase) decreased after BF treatment; sucrase (sucrose alpha-glucosidase) activity was not affected. On the other hand, in B10.BR mice, specific activity of gamma-glutamyltransferase and dipeptidyl peptidase IV were higher after administration of BF in both CV and GF groups relative to untreated controls. The activities of lactase and glucoamylase (glucan 1,4-alpha-glucosidase) were significantly stimulated only in the group of GF B10.BR mice treated with formolized BF. The stimulation of immunoglobulin production after BF treatment was accompanied by changes in the levels of enterocyte brush-border enzymes; this responsiveness to BF treatment was genetically regulated.
See more in PubMed
Infect Immun. 2001 Apr;69(4):2372-7 PubMed
Biochim Biophys Acta. 1972 Sep 19;284(1):235-47 PubMed
Anal Biochem. 1976 Aug;74(2):466-76 PubMed
Semin Immunol. 1996 Feb;8(1):11-8 PubMed
Folia Microbiol (Praha). 1997;42(4):403-8 PubMed
Parasitol Res. 2001 Jul;87(7):570-2 PubMed
J Immunol. 1999 May 1;162(9):5112-8 PubMed
Folia Microbiol (Praha). 1994;39(2):147-51 PubMed
Infect Immun. 1995 Oct;63(10):3904-13 PubMed
Anal Biochem. 1964 Jan;7:18-25 PubMed
J Reprod Immunol. 1989 Jul;15(3):217-27 PubMed
Folia Microbiol (Praha). 2002;47(2):193-7 PubMed
Biochem Biophys Res Commun. 1976 Jul 12;71(1):32-6 PubMed
Biochim Biophys Acta. 1978 Jan 4;506(1):136-54 PubMed
Biochim Biophys Acta. 1972 Feb 28;258(2):520-30 PubMed
Int J Immunopharmacol. 1994 May-Jun;16(5-6):487-93 PubMed
Folia Microbiol (Praha). 1995;40(4):413-6 PubMed
Gastroenterology. 1994 Feb;106(2):533-9 PubMed
Gut. 1999 Jan;44(1):2-5 PubMed
Int J Immunopharmacol. 1998 Jul;20(7):359-68 PubMed
Springer Semin Immunopathol. 1997;18(4):449-61 PubMed
Endocrine. 1997 Apr;6(2):187-94 PubMed
J Biol Chem. 1996 Jan 12;271(2):1237-42 PubMed
Folia Microbiol (Praha). 1992;37(6):455-60 PubMed
J Immunol. 1985 Nov;135(5):3277-83 PubMed
Folia Microbiol (Praha). 1994;39(6):501-4 PubMed
J Biol Chem. 1993 Jul 5;268(19):14116-24 PubMed
Folia Microbiol (Praha). 1995;40(6):647-51 PubMed
