Phospholipase D (PLD) and its product phosphatidic acid (PA) are involved in a number of signalling pathways regulating cell proliferation, membrane vesicle trafficking and defence responses in eukaryotic cells. Here we report that PLD and PA have a role in the process of polarised plant cell expansion as represented by pollen tube growth. Both phosphatidylinositol-4,5-bisphosphate-dependent and independent PLD activities were identified in pollen tube extracts, and activity levels during pollen tube germination and growth were measured. PLD-mediated PA production in vivo can be blocked by primary alcohols, which serve as a substrate for the transphosphatidylation reaction. Both pollen germination and tube growth are stopped in the presence 0.5% 1-butanol, whereas secondary and tertiary isomers do not show any effect. This inhibition could be overcome by addition of exogenous PA-containing liposomes. In the absence of n-butanol, addition of a micromolar concentration of PA specifically stimulates pollen germination and tube elongation. Furthermore, a recently established link between PLD and microtubule dynamics was supported by taxol-mediated partial rescue of the 1-butanol-inhibited pollen tubes. The potential signalling role for PLD-derived PA in plant cell expansion is discussed.

Department of Plant Physiology Faculty of Science Charles University Vinicná 5 128 44 Prague 2 Czech Republic

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Plant Cell. 2001 Sep;13(9):2143-58 PubMed

Biochim Biophys Acta. 2001 Feb 26;1530(2-3):172-83 PubMed

Biochem J. 2000 Feb 1;345 Pt 3:401-15 PubMed

BMC Genomics. 2002;3:2 PubMed

Plant Cell. 1996 Jun;8(6):1079-1090 PubMed

J Biol Chem. 1997 Mar 14;272(11):7055-61 PubMed

FEBS Lett. 2002 Mar 27;515(1-3):39-43 PubMed

J Biol Chem. 2001 Jul 27;276(30):28252-60 PubMed

Trends Plant Sci. 2001 May;6(5):227-33 PubMed

Dev Biol. 1996 Feb 25;174(1):160-73 PubMed

J Microsc. 2000 Jun;198(Pt 3):218-45 PubMed

Plant Cell. 1997 Dec;9(12):2183-96 PubMed

Arch Biochem Biophys. 1999 Aug 15;368(2):347-53 PubMed

Prog Lipid Res. 2000 Mar;39(2):109-49 PubMed

Biochem Biophys Res Commun. 1985 Aug 15;130(3):1241-6 PubMed

Proc Natl Acad Sci U S A. 1998 Mar 3;95(5):2697-702 PubMed

J Biol Chem. 2002 Jan 25;277(4):3030-9 PubMed

J Biol Chem. 2000 Jun 30;275(26):19700-6 PubMed

J Cell Biol. 1999 Apr 19;145(2):317-30 PubMed

J Biol Chem. 1994 Apr 15;269(15):11547-54 PubMed

J Biol Chem. 1997 Nov 7;272(45):28267-73 PubMed

Biochem Mol Biol Int. 1997 Apr;41(4):715-24 PubMed

Mol Biol Cell. 2001 Apr;12(4):943-55 PubMed

Arch Biochem Biophys. 1998 May 1;353(1):131-40 PubMed

Plant Cell. 1996 Aug;8(8):1305-1321 PubMed

Curr Opin Plant Biol. 2002 Aug;5(4):332-8 PubMed

Plant Physiol. 1999 Apr;119(4):1371-8 PubMed

Plant Physiol. 1996 May;111(1):101-107 PubMed

Plant Mol Biol. 1995 Jul;28(4):739-50 PubMed

Plant J. 2001 Jan;25(1):55-65 PubMed

J Cell Sci. 1997 Jun;110 ( Pt 11):1269-78 PubMed

Plant Physiol. 2002 Aug;129(4):1507-20 PubMed

Plant Cell. 2000 Nov;12(11):2237-46 PubMed

Plant Cell. 2000 Jan;12(1):111-24 PubMed

Nature. 2000 Dec 14;408(6814):796-815 PubMed

J Biol Chem. 1993 Oct 15;268(29):21509-12 PubMed

Biochim Biophys Acta. 1998 Jan 23;1389(3):222-72 PubMed

Annu Rev Cell Dev Biol. 2001;17:159-87 PubMed

Plant Cell. 1995 Dec;7(12):2197-2210 PubMed

Cell Mol Life Sci. 2001 Oct;58(11):1674-87 PubMed

J Neurochem. 2001 Dec;79(5):923-30 PubMed

J Cell Biol. 1996 Jul;134(2):295-306 PubMed

Curr Biol. 1997 May 1;7(5):301-7 PubMed

EMBO J. 2000 Oct 16;19(20):5440-9 PubMed

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