Isolation of Arcobacter butzleri and A. cryaerophilus in samples of meats and from meat-processing plants by a culture technique and detection by PCR
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu srovnávací studie, hodnotící studie, časopisecké články, práce podpořená grantem
PubMed
12800507
DOI
10.1007/bf02930960
Knihovny.cz E-zdroje
- MeSH
- agar MeSH
- Arcobacter klasifikace genetika růst a vývoj izolace a purifikace MeSH
- DNA bakterií analýza MeSH
- kontaminace potravin MeSH
- kontaminace zdravotnického vybavení MeSH
- kultivační média MeSH
- kur domácí mikrobiologie MeSH
- masné výrobky mikrobiologie MeSH
- maso mikrobiologie MeSH
- polymerázová řetězová reakce metody MeSH
- prasata mikrobiologie MeSH
- průmysl zpracování potravin * MeSH
- skot MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- agar MeSH
- DNA bakterií MeSH
- kultivační média MeSH
A pilot survey of sources of contamination with arcobacters (representing a potential risk for humans) was done in a wide range of samples involved various kinds of meat (beef, pork, meat products, chilled chickens, etc.) from a retail level and domestic farming. Sanitary practices in slaughterhouses and production lines were checked in two different plants (a beef and pork production and a chicken processing plant). The method is based on a selective enrichment to isolate suspect strains, in combination with a PCR technique specific for arcobacters. The choice of a suitable enrichment broth and a plating agar was made with the use of pure bacterial strains and by means of real meat samples seeded with Arcobacter butzleri. The PCR technique was optimized to allow differentiation of a 1223 bp product, typical of the genus Arcobacter, and a product of 686 bp, specific for A. butzleri a total number of 198 samples were tested, of that 33 (17%) were found to be positive for the genus Arcobacter but only 22 (11%) for A. butzleri.
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Identification of Arcobacter species using phospholipid and total fatty acid profiles
