Isolation of Arcobacter butzleri and A. cryaerophilus in samples of meats and from meat-processing plants by a culture technique and detection by PCR
Language English Country United States Media print
Document type Comparative Study, Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
12800507
DOI
10.1007/bf02930960
Knihovny.cz E-resources
- MeSH
- Agar MeSH
- Arcobacter classification genetics growth & development isolation & purification MeSH
- DNA, Bacterial analysis MeSH
- Food Contamination MeSH
- Equipment Contamination MeSH
- Culture Media MeSH
- Chickens microbiology MeSH
- Meat Products microbiology MeSH
- Meat microbiology MeSH
- Polymerase Chain Reaction methods MeSH
- Swine microbiology MeSH
- Food-Processing Industry * MeSH
- Cattle MeSH
- Animals MeSH
- Check Tag
- Cattle MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Agar MeSH
- DNA, Bacterial MeSH
- Culture Media MeSH
A pilot survey of sources of contamination with arcobacters (representing a potential risk for humans) was done in a wide range of samples involved various kinds of meat (beef, pork, meat products, chilled chickens, etc.) from a retail level and domestic farming. Sanitary practices in slaughterhouses and production lines were checked in two different plants (a beef and pork production and a chicken processing plant). The method is based on a selective enrichment to isolate suspect strains, in combination with a PCR technique specific for arcobacters. The choice of a suitable enrichment broth and a plating agar was made with the use of pure bacterial strains and by means of real meat samples seeded with Arcobacter butzleri. The PCR technique was optimized to allow differentiation of a 1223 bp product, typical of the genus Arcobacter, and a product of 686 bp, specific for A. butzleri a total number of 198 samples were tested, of that 33 (17%) were found to be positive for the genus Arcobacter but only 22 (11%) for A. butzleri.
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