Cleavage of double stranded plasmid DNA by lanthanide complexes
Language English Country Netherlands Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
14698251
DOI
10.1016/j.jchromb.2003.09.011
PII: S1570023203007281
Knihovny.cz E-resources
- MeSH
- Chromosomes chemistry MeSH
- Deoxyribonuclease EcoRI chemistry MeSH
- DNA chemistry MeSH
- Edetic Acid MeSH
- Electrophoresis, Agar Gel MeSH
- Indicators and Reagents MeSH
- Lanthanoid Series Elements chemistry MeSH
- Methacrylates MeSH
- Plasmids chemistry MeSH
- Pyridines chemistry MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Deoxyribonuclease EcoRI MeSH
- DNA MeSH
- Edetic Acid MeSH
- Indicators and Reagents MeSH
- Lanthanoid Series Elements MeSH
- Methacrylates MeSH
- pyridine MeSH Browser
- Pyridines MeSH
The use of free lanthanide ions and their complexes for plasmid DNA pBR322 and chromosomal DNA cleavage was studied. Plasmid pBR322 DNA was treated by lanthanide chlorides (Eu(3+), La(3+), Nd(3+), Pr(3+), Gd(3+)) in HEPES buffer (pH 7.0, 7.5 and 8.0) at 24, 37, 50, 63, and 76 degrees C. The formation of linear and nicked plasmid forms was investigated depending on the reaction conditions. Heterogeneous lanthanide complexes of ethylenediamine tetraacetic acid (EDTA) immobilized on insoluble methacrylate support and iminodiacetic acid (IDA) immobilized on styrene support were used as catalysts plasmid for DNA pBR322 cleavage, too. The temperature of reaction mixture had substantial influence on cleavage rate. The precipitation of DNA occurred during the measurement of interactions between chromosomal DNA and La(3+) ions.
References provided by Crossref.org
