Involvement of filamentous actin in setting the threshold for degranulation in mast cells
Language English Country Germany Media print
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
15162432
DOI
10.1002/eji.200424991
Knihovny.cz E-resources
- MeSH
- Receptor Aggregation immunology MeSH
- Actins immunology physiology MeSH
- Antigens, Surface immunology MeSH
- Thy-1 Antigens immunology MeSH
- Bridged Bicyclo Compounds, Heterocyclic pharmacology MeSH
- Cell Degranulation immunology physiology MeSH
- Phospholipase C gamma MeSH
- Type C Phospholipases immunology MeSH
- Phosphoproteins immunology MeSH
- Phosphorylation MeSH
- Intracellular Signaling Peptides and Proteins MeSH
- Syk Kinase MeSH
- Rats MeSH
- Mast Cells cytology immunology physiology MeSH
- Membrane Glycoproteins immunology MeSH
- Membrane Proteins immunology MeSH
- Cell Line, Tumor MeSH
- Enzyme Precursors immunology MeSH
- Receptors, IgE immunology MeSH
- Signal Transduction immunology MeSH
- Thiazolidines MeSH
- Thiazoles pharmacology MeSH
- Carrier Proteins immunology MeSH
- Protein-Tyrosine Kinases immunology MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Actins MeSH
- Antigens, Surface MeSH
- Thy-1 Antigens MeSH
- Bridged Bicyclo Compounds, Heterocyclic MeSH
- Phospholipase C gamma MeSH
- Type C Phospholipases MeSH
- Phosphoproteins MeSH
- Intracellular Signaling Peptides and Proteins MeSH
- Syk Kinase MeSH
- latrunculin B MeSH Browser
- Membrane Glycoproteins MeSH
- Membrane Proteins MeSH
- Enzyme Precursors MeSH
- Receptors, IgE MeSH
- Syk protein, rat MeSH Browser
- TEC-21 antigen, rat MeSH Browser
- Thiazolidines MeSH
- Thiazoles MeSH
- Carrier Proteins MeSH
- Protein-Tyrosine Kinases MeSH
Previous studies using cytochalasins and latrunculin B, inhibitors of actin polymerization, showed that filamentous (F)-actin had a negative regulatory role in Fc epsilon receptor I (Fc epsilon RI) signaling. How F-actin is involved in regulating the activation of mast cells is unknown. In this study we investigated the role of F-actin in mast cell activation induced by aggregation of the glycosylphosphatidylinositol (GPI)-anchored proteins Thy-1 and TEC-21, and compared it to activation via Fc epsilon RI. Pretreatment of rat basophilic leukemia cells with latrunculin B inhibited the Thy-1-induced actin polymerization and elevated the Thy-1-mediated secretory and calcium responses. Inhibition of actin polymerization followed by Thy-1 aggregation resulted in an increased tyrosine phosphorylation of Syk, phospholipase C gamma (PLC gamma), Gab2 and linker for activation of T cells (LAT) adapters, and some other signaling molecules. Enzymatic activities of phosphatidylinositol 3-kinase, PLC gamma, and phosphatase SHP-2 were also up-regulated, but tyrosine phosphorylation of ezrin was inhibited. Similar changes were observed in Fc epsilon RI-activated cells. Significant changes in intracellular distribution, tyrosine phosphorylation, and/or enzymatic activities of signaling molecules occurred in latrunculin-pretreated cells before cell triggering. The combined data suggest that actin polymerization is critical for setting the thresholds for mast cell signaling via aggregation of both Fc epsilon RI and GPI-anchored proteins.
References provided by Crossref.org
New Regulatory Roles of Galectin-3 in High-Affinity IgE Receptor Signaling
Cytoskeleton in mast cell signaling
Negative regulation of mast cell signaling and function by the adaptor LAB/NTAL
