Activation of pig oocytes using nitric oxide donors
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
15736132
DOI
10.1002/mrd.20248
Knihovny.cz E-resources
- MeSH
- Nitric Oxide Donors pharmacology MeSH
- Enzyme Inhibitors pharmacology MeSH
- Calmodulin metabolism MeSH
- Cells, Cultured MeSH
- NG-Nitroarginine Methyl Ester pharmacology MeSH
- Nitroprusside pharmacology MeSH
- Oocytes cytology drug effects metabolism MeSH
- Nitric Oxide metabolism MeSH
- Penicillamine analogs & derivatives pharmacology MeSH
- Swine * MeSH
- Nitric Oxide Synthase Type III MeSH
- Nitric Oxide Synthase antagonists & inhibitors metabolism MeSH
- In Vitro Techniques MeSH
- Animals MeSH
- Check Tag
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Nitric Oxide Donors MeSH
- Enzyme Inhibitors MeSH
- Calmodulin MeSH
- NG-Nitroarginine Methyl Ester MeSH
- Nitroprusside MeSH
- Nitric Oxide MeSH
- Penicillamine MeSH
- S-nitro-N-acetylpenicillamine MeSH Browser
- Nitric Oxide Synthase Type III MeSH
- Nitric Oxide Synthase MeSH
Nitric oxide (NO) plays an important role in intracellular signaling, but its role during the activation of mammalian oocytes is little understood. In our study, in vitro matured pig oocytes were cultured with NO-donors-S-nitroso-N-acetylpenicillamine (SNAP) or sodium nitropruside (SNP). These treatments were able to induce parthenogenetic activation of pig oocytes matured in vitro. The specificity of this effect was confirmed by the activation of oocytes by exogenous endothelial nitric oxide synthase (eNOS) microinjected in the oocyte with its activator calmodulin. Relatively long exposure (10 hr) is needed for activation of pig oocytes with 2.0 mM SNAP. An active NOS is necessary for the NO-dependent activation of pig oocytes because NOS inhibitors L-NMMA or L-NAME are able to inhibit activation of oocytes with NO-donor SNAP. On the basis of our data, we conclude that the NO-dependent activating stimulus seems inadequate because it did not induce the exocytosis of cortical granules. Also, the cleavage of parthenogenetic embryos was very low, and embryos did not develop beyond the stage of eight blastomeres.
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