Sample processing effect on polymerase chain reaction used for identification of Campylobacter jejuni
Language English Country United States Media print
Document type Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
15881405
DOI
10.1007/bf02931551
Knihovny.cz E-resources
- MeSH
- Campylobacter coli classification genetics isolation & purification MeSH
- Campylobacter jejuni classification genetics isolation & purification MeSH
- Campylobacter classification genetics isolation & purification MeSH
- Sodium Chloride MeSH
- Culture Media MeSH
- Humans MeSH
- Polymerase Chain Reaction methods MeSH
- Food Microbiology * MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Sodium Chloride MeSH
- Culture Media MeSH
Model samples of Campylobacter jejuni for polymerase chain reaction (PCR) were prepared by rapid and simple procedures consisting of centrifugation, proteinase K treatment, Chelex 100 treatment, and boiling lyses. A PCR based on specific amplification of the variable sequence of 16S rRNA gene was performed using Tth DNA polymerase and the PCR products were visualized by agarose gel electrophoresis. The assay allowed the detection of 10 CFU/mL C. jejuni in the physiological saline and 100 CFU/mL in the basic Park and Sanders broth.
Department of Biochemistry and Microbiology Institute of Chemical Technology 166 28 Prague 6 Czechia
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