Quantitative detection of species-specific DNA in feedstuffs and fish meals
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
15954711
DOI
10.4315/0362-028x-68.6.1217
PII: S0362-028X(22)01062-6
Knihovny.cz E-resources
- MeSH
- Species Specificity MeSH
- Animal Feed analysis MeSH
- Chickens genetics MeSH
- DNA, Mitochondrial analysis MeSH
- Sheep genetics MeSH
- Polymerase Chain Reaction methods MeSH
- Swine genetics MeSH
- Ruminants * MeSH
- Prion Diseases prevention & control MeSH
- Fish Products analysis MeSH
- Sensitivity and Specificity MeSH
- Cattle genetics MeSH
- Animals MeSH
- Check Tag
- Cattle genetics MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA, Mitochondrial MeSH
A sensitive and rapid method for the quantitative detection of bovine-, ovine-, swine-, and chicken-specific mitochondrial DNA sequences based on real-time PCR has been developed. The specificity of the primers and probes for real-time PCR has been tested using DNA samples of other vertebrate species that may also be present in rendered products. The quantitative detection was performed with dual-labeled probes (TaqMan) using absolute quantification with external standards of single species meat-and-bone meals. This method facilitates the detection of 0.01% of the target species-derived material in concentrate feed mixtures and fish meals.
References provided by Crossref.org
Quantitative real-time PCR study on persistence of pDNA vaccine pVax-Hsp60 TM814 in beef muscles
