Quantitative detection of species-specific DNA in feedstuffs and fish meals

. 2005 Jun ; 68 (6) : 1217-21.

Jazyk angličtina Země Spojené státy americké Médium print

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/pmid15954711
Odkazy

PubMed 15954711
DOI 10.4315/0362-028x-68.6.1217
PII: S0362-028X(22)01062-6
Knihovny.cz E-zdroje

A sensitive and rapid method for the quantitative detection of bovine-, ovine-, swine-, and chicken-specific mitochondrial DNA sequences based on real-time PCR has been developed. The specificity of the primers and probes for real-time PCR has been tested using DNA samples of other vertebrate species that may also be present in rendered products. The quantitative detection was performed with dual-labeled probes (TaqMan) using absolute quantification with external standards of single species meat-and-bone meals. This method facilitates the detection of 0.01% of the target species-derived material in concentrate feed mixtures and fish meals.

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