Genotypic characterization of toxic shock syndrome toxin-1-producing strains of Staphylococcus aureus isolated in the Czech Republic
Language English Country Germany Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
16423687
DOI
10.1016/j.ijmm.2005.07.014
PII: S1438-4221(05)00142-6
Knihovny.cz E-resources
- MeSH
- Bacterial Toxins biosynthesis genetics MeSH
- DNA, Bacterial chemistry genetics MeSH
- DNA Fingerprinting MeSH
- Enterotoxins biosynthesis genetics MeSH
- Genotype MeSH
- DNA, Intergenic chemistry genetics MeSH
- Humans MeSH
- Polymerase Chain Reaction MeSH
- Shock, Septic microbiology MeSH
- Staphylococcal Infections microbiology MeSH
- Staphylococcus aureus genetics isolation & purification metabolism MeSH
- Superantigens biosynthesis genetics MeSH
- Check Tag
- Humans MeSH
- Male MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czech Republic MeSH
- Names of Substances
- Bacterial Toxins MeSH
- DNA, Bacterial MeSH
- enterotoxin F, Staphylococcal MeSH Browser
- Enterotoxins MeSH
- DNA, Intergenic MeSH
- Superantigens MeSH
Genotyping based on pulsed-field gel electrophoresis (PFGE), PCR ribotyping, enterobacterial repetitive intergenic consensus motif (ERIC2)-PCR, and prophage content analysis was carried out in 45 toxic shock syndrome toxin-1 (TSST-1)-positive Staphylococcus aureus strains isolated in 19 cities of the Czech Republic between 1986 and 2004. Twenty-four wild-type TSST-1-positive isolates of which 18 strains were the causative agents of toxic shock syndrome (TSS) had the same genotype profiles as the type strain FRI 1169 and were classified into genotype T1. The remaining 21 strains of which 12 caused TSS were distinct from one another and differed from the T1 strains mainly in PFGE pattern and also in one or more other genetic characteristics. While the strains of genotype T1 are most prevalent in the Czech population and account for the majority of TSS cases, TSS-related S. aureus strains of different genotypes were also found and cannot be excluded as the causative agents of TSS cases in this country.
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