Effect of free radical spin trap N-tert-butyl-alpha-phenylnitrone (PBN) on seizures induced in immature rats by homocysteic acid
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
16756975
DOI
10.1016/j.expneurol.2006.03.031
PII: S0014-4886(06)00243-3
Knihovny.cz E-resources
- MeSH
- Adenosine Triphosphate metabolism MeSH
- Disaccharides administration & dosage pharmacology MeSH
- Electroencephalography MeSH
- Energy Metabolism drug effects MeSH
- Phosphocreatine metabolism MeSH
- Glucose metabolism MeSH
- Glycogen metabolism MeSH
- Hippocampus drug effects metabolism pathology MeSH
- Homocysteine analogs & derivatives toxicity MeSH
- Injections, Intraperitoneal MeSH
- Rats MeSH
- Brain drug effects metabolism pathology MeSH
- Cerebral Cortex drug effects metabolism pathology MeSH
- Neurons drug effects metabolism pathology MeSH
- Rats, Wistar MeSH
- Spin Trapping MeSH
- Free Radicals antagonists & inhibitors MeSH
- Seizures chemically induced physiopathology prevention & control MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Adenosine Triphosphate MeSH
- Disaccharides MeSH
- Phosphocreatine MeSH
- Glucose MeSH
- Glycogen MeSH
- homocysteic acid MeSH Browser
- Homocysteine MeSH
- PBNLP compound MeSH Browser
- Free Radicals MeSH
The present study has examined the effect of free radical spin trap N-tert-butyl-alpha-phenylnitrone (PBN) in the model of seizures induced in immature 12-day-old rats by bilateral intracerebroventricular infusion of dl-homocysteic acid (dl-HCA, 600 nmol/side). PBN was given i.p. in two doses (100 mg/kg each), 30 min prior and 30 min after dl-HCA infusion. PBN did not significantly influence the severity of seizures, evident both from the behavioral symptoms and EEG recordings. PBN normalized decreased ATP levels in the hippocampus, occurring during the acute phase of seizures ( approximately 45-50 min after infusion) and persisting until the end of the 24-h recovery period. PBN also led to normalization of decreased glucose levels and to a significant reduction of lactate accumulation in the cerebral cortex and hippocampus. The neuroprotective effect of PBN was evaluated after 24 h and 6 days of survival following dl-HCA-induced seizures (Nissl and Fluoro-Jade B staining). The administration of PBN resulted in a partial amelioration of severe damage observed in many brain regions following infusion of dl-HCA alone. The data suggest that increased free radical production is apparently occurring during seizures induced in immature rats by homocysteic acid. Free radical scavenger PBN had a clear-cut protective effect, evident as the improved recovery of brain energy status and as a partial, but significant, attenuation of neuronal degeneration associated with this model of seizures.
References provided by Crossref.org
