Localization of pyruvate:NADP+ oxidoreductase in sporozoites of Cryptosporidium parvum
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, Research Support, N.I.H., Extramural, práce podpořená grantem
Grantová podpora
1R03 TW01507-01
FIC NIH HHS - United States
5D43 TW00915-05
FIC NIH HHS - United States
PubMed
16872290
DOI
10.1111/j.1550-7408.2006.00099.x
PII: JEU99
Knihovny.cz E-zdroje
- MeSH
- Cryptosporidium parvum cytologie enzymologie genetika růst a vývoj MeSH
- cytosol enzymologie MeSH
- Euglena gracilis cytologie enzymologie MeSH
- fluorescenční mikroskopie MeSH
- ketonoxidoreduktasy analýza genetika imunologie MeSH
- konfokální mikroskopie MeSH
- NADPH-cytochrom c-reduktasa analýza genetika imunologie MeSH
- organely enzymologie MeSH
- protozoální proteiny analýza MeSH
- pyruvátsynthasa analýza genetika imunologie MeSH
- sporozoiti cytologie enzymologie genetika MeSH
- transmisní elektronová mikroskopie MeSH
- western blotting MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, N.I.H., Extramural MeSH
- Názvy látek
- ketonoxidoreduktasy MeSH
- NADPH-cytochrom c-reduktasa MeSH
- protozoální proteiny MeSH
- pyruvate dehydrogenase (NADP+) MeSH Prohlížeč
- pyruvátsynthasa MeSH
Cryptosporidium parvum contains a unique fusion protein pyruvate:NADP+ oxidoreductase (CpPNO) that is composed of two distinct, conserved domains, an N-terminal pyruvate:ferredoxin oxidoreductase (PFO) and a C-terminal cytochrome P450 reductase (CPR). Unlike a similar fusion protein that localizes to the mitochondrion of the photosynthetic protist Euglena gracilis, CpPNO lacks an N-terminal mitochondrial targeting sequence. Using two distinct polyclonal antibodies raised against CpPFO and one polyclonal antibody against CpCPR, Western blot analysis has shown that sporozoites of C. parvum express the entire CpPNO fusion protein. Furthermore, confocal immunofluorescence and transmission electron microscopy confirm that CpPNO is localized within the cytosol rather than the relict mitochondrion of C. parvum. The distribution of this protein is not, however, strictly confined to the cytosol. CpPNO also appears to localize posteriorly within the crystalloid body.
Citace poskytuje Crossref.org
