Molecular mechanisms of insulin-like growth factor 1 promoted synthesis and retention of hyaluronic acid in porcine oocyte-cumulus complexes
Jazyk angličtina Země Spojené státy americké Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
17329596
DOI
10.1095/biolreprod.106.057927
PII: biolreprod.106.057927
Knihovny.cz E-zdroje
- MeSH
- AMP cyklický biosyntéza MeSH
- folikulární buňky cytologie účinky léků metabolismus MeSH
- folikuly stimulující hormon farmakologie MeSH
- fosfatidylinositol-3-kinasy metabolismus MeSH
- fosforylace účinky léků MeSH
- glukuronosyltransferasa genetika metabolismus MeSH
- hyaluronansynthasy MeSH
- insulinu podobný růstový faktor I farmakologie MeSH
- kultivované buňky MeSH
- kyselina hyaluronová biosyntéza metabolismus MeSH
- mitogenem aktivovaná proteinkinasa 3 metabolismus MeSH
- oocyty účinky léků metabolismus MeSH
- prasata MeSH
- proliferace buněk účinky léků MeSH
- proteinkinasy závislé na cyklickém AMP metabolismus MeSH
- protoonkogenní proteiny c-akt metabolismus MeSH
- zvířata MeSH
- Check Tag
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- AMP cyklický MeSH
- folikuly stimulující hormon MeSH
- fosfatidylinositol-3-kinasy MeSH
- glukuronosyltransferasa MeSH
- hyaluronansynthasy MeSH
- insulinu podobný růstový faktor I MeSH
- kyselina hyaluronová MeSH
- mitogenem aktivovaná proteinkinasa 3 MeSH
- proteinkinasy závislé na cyklickém AMP MeSH
- protoonkogenní proteiny c-akt MeSH
The purpose of the present study was to elucidate signaling pathways by which insulin like-growth factor 1 (IGF1) promotes FSH-stimulated synthesis and retention of hyaluronic acid (HA) in pig oocyte-cumulus complexes (OCCs) cultured in serum-free medium. We found that IGF1 had no effects on FSH-stimulated production of cAMP and activation of protein kinase A in the OCCs. Immunoblotting with phospho-specific antibodies showed that FSH moderately phosphorylated v-akt murine thymoma viral oncogene homolog (AKT) and mitogen-activated kinase 3 and 1 (MAPK3/1) in cumulus cells. The exposure of OCCs to both FSH and IGF1 resulted in a significant (P < 0.05) increase in AKT and MAPK3/1 phosphorylation. An inhibitor of phosphoinositide-3-kinase (PIK3), LY 294002, significantly (P < 0.05) reduced the IGF1-enhanced phosphorylation of AKT, and inhibitors of AKT (SH6) and MAPK3/1 (U0126) significantly (P < 0.05) decreased the synthesis and retention of HA stimulated by concomitant exposure of OCCs to both FSH and IGF1. The IGF1-promoted synthesis of HA was not accompanied by an increase in the relative abundance of hyaluronan synthase 2 (HAS2) mRNA in the cumulus cells. We conclude that IGF1 promotes the FSH-stimulated synthesis and retention of HA in pig OCCs by PIK3/AKT- and MAPK3/1-dependent mechanisms.
Citace poskytuje Crossref.org
A Role of PI3K/Akt Signaling in Oocyte Maturation and Early Embryo Development
Regulation of mitogen-activated protein kinase 3/1 activity during meiosis resumption in mammals
