Molecular mechanisms of insulin-like growth factor 1 promoted synthesis and retention of hyaluronic acid in porcine oocyte-cumulus complexes
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
17329596
DOI
10.1095/biolreprod.106.057927
PII: biolreprod.106.057927
Knihovny.cz E-resources
- MeSH
- Cyclic AMP biosynthesis MeSH
- Granulosa Cells cytology drug effects metabolism MeSH
- Follicle Stimulating Hormone pharmacology MeSH
- Phosphatidylinositol 3-Kinases metabolism MeSH
- Phosphorylation drug effects MeSH
- Glucuronosyltransferase genetics metabolism MeSH
- Hyaluronan Synthases MeSH
- Insulin-Like Growth Factor I pharmacology MeSH
- Cells, Cultured MeSH
- Hyaluronic Acid biosynthesis metabolism MeSH
- Mitogen-Activated Protein Kinase 3 metabolism MeSH
- Oocytes drug effects metabolism MeSH
- Swine MeSH
- Cell Proliferation drug effects MeSH
- Cyclic AMP-Dependent Protein Kinases metabolism MeSH
- Proto-Oncogene Proteins c-akt metabolism MeSH
- Animals MeSH
- Check Tag
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Cyclic AMP MeSH
- Follicle Stimulating Hormone MeSH
- Phosphatidylinositol 3-Kinases MeSH
- Glucuronosyltransferase MeSH
- Hyaluronan Synthases MeSH
- Insulin-Like Growth Factor I MeSH
- Hyaluronic Acid MeSH
- Mitogen-Activated Protein Kinase 3 MeSH
- Cyclic AMP-Dependent Protein Kinases MeSH
- Proto-Oncogene Proteins c-akt MeSH
The purpose of the present study was to elucidate signaling pathways by which insulin like-growth factor 1 (IGF1) promotes FSH-stimulated synthesis and retention of hyaluronic acid (HA) in pig oocyte-cumulus complexes (OCCs) cultured in serum-free medium. We found that IGF1 had no effects on FSH-stimulated production of cAMP and activation of protein kinase A in the OCCs. Immunoblotting with phospho-specific antibodies showed that FSH moderately phosphorylated v-akt murine thymoma viral oncogene homolog (AKT) and mitogen-activated kinase 3 and 1 (MAPK3/1) in cumulus cells. The exposure of OCCs to both FSH and IGF1 resulted in a significant (P < 0.05) increase in AKT and MAPK3/1 phosphorylation. An inhibitor of phosphoinositide-3-kinase (PIK3), LY 294002, significantly (P < 0.05) reduced the IGF1-enhanced phosphorylation of AKT, and inhibitors of AKT (SH6) and MAPK3/1 (U0126) significantly (P < 0.05) decreased the synthesis and retention of HA stimulated by concomitant exposure of OCCs to both FSH and IGF1. The IGF1-promoted synthesis of HA was not accompanied by an increase in the relative abundance of hyaluronan synthase 2 (HAS2) mRNA in the cumulus cells. We conclude that IGF1 promotes the FSH-stimulated synthesis and retention of HA in pig OCCs by PIK3/AKT- and MAPK3/1-dependent mechanisms.
References provided by Crossref.org
A Role of PI3K/Akt Signaling in Oocyte Maturation and Early Embryo Development
Regulation of mitogen-activated protein kinase 3/1 activity during meiosis resumption in mammals
