The involvement of the PsbI protein in the assembly and repair of the photosystem II (PSII) complex has been studied in the cyanobacterium Synechocystis sp. PCC 6803. Analysis of PSII complexes in the wild-type strain showed that the PsbI protein was present in dimeric and monomeric core complexes, core complexes lacking CP43, and in reaction center complexes containing D1, D2, and cytochrome b-559. In addition, immunoprecipitation experiments and the use of a histidine-tagged derivative of PsbI have revealed the presence in the thylakoid membrane of assembly complexes containing PsbI and either the precursor or mature forms of D1. Analysis of PSII assembly in the psbI deletion mutant and in strains lacking PsbI together with other PSII subunits showed that PsbI was not required for formation of PSII reaction center complexes or core complexes, although levels of unassembled D1 were reduced in its absence. However, loss of PsbI led to a dramatic destabilization of CP43 binding within monomeric and dimeric PSII core complexes. Despite the close structural relationship between D1 and PsbI in the PSII complex, PsbI turned over much slower than D1, whereas high light-induced turnover of D1 was accelerated in the absence of PsbI. Overall, our results suggest that PsbI is an early assembly partner for D1 and that it plays a functional role in stabilizing the binding of CP43 in the PSII holoenzyme.

Institute of Microbiology Academy of Sciences 37981 Trebon Czech Republic

Zobrazit více v PubMed

Anbudurai PR, Mor TS, Ohad I, Shestakov SV, Pakrasi HB (1994) The CtpA gene encodes the C-terminal processing protease for the D1 protein of the photosystem-II reaction-center complex. Proc Natl Acad Sci USA 91 8082–8086 PubMed PMC

Barber J (2006) Photosystem II: an enzyme of global significance. Biochem Soc Trans 34 619–631 PubMed

Bumba L, Tichý M, Dobáková M, Komenda J, Vácha F (2005) Localization of the PsbH subunit in photosystem II from the Synechocystis 6803 using the His-tagged Ni-NTA nanogold labeling. J Struct Biol 152 28–35 PubMed

Burke E, Barik S (2003) Megaprimer PCR: application in mutagenesis and gene fusion. Methods Mol Biol 226 525–532 PubMed

Eaton-Rye JJ, Vermaas WFJ (1991) Oligonucleotide-directed mutagenesis of psbB, the gene encoding CP47, employing a deletion mutant strain of the cyanobacterium Synechocystis sp. PCC 6803. Plant Mol Biol 17 1165–1177 PubMed

Ferreira KN, Iverson TN, Maglaoui K, Barber J, Iwata S (2004) Architecture of the photosynthetic oxygen-evolving center. Science 303 1831–1838 PubMed

Ikeuchi M, Koike H, Inoue Y (1989) Identification of psbI and psbL gene products in cyanobacteria photosystem II reaction center preparation. FEBS Lett 251 155–160 PubMed

Ikeuchi M, Shukla VK, Pakrasi HB, Inoue Y (1995) Directed inactivation of the psbI gene does not affect photosystem II in the cyanobacterium Synechocystis sp. PCC 6803. Mol Gen Genet 249 622–628 PubMed

Inagaki N, Yamamoto Y, Satoh K (2001) A sequential two-step proteolytic process in the carboxyl-terminal truncation of precursor D1 protein in Synechocystis sp. PCC 6803. FEBS Lett 509 197–201 PubMed

Komenda J, Barber J (1995) Comparison of psbO and psbH deletion mutants of Synechocystis PCC 6803 indicates that degradation of D1 protein is regulated by the QB site and is dependent on protein synthesis. Biochemistry 34 9625–9631 PubMed

Komenda J, Barker M, Kuviková S, DeVries R, Mullineaux CW, Tichý M, Nixon PJ (2006) The FtsH protease slr0228 is important for quality control of photosystem II in the thylakoid membrane of Synechocystis PCC 6803. J Biol Chem 281 1145–1151 PubMed

Komenda J, Kuviková S, Granvogl B, Eichacker LA, Diner BA, Nixon PJ (2007) Cleavage after residue Ala352 in the C-terminal extension is an early step in the maturation of the D1 subunit of photosystem II in Synechocystis PCC 6803. Biochim Biophys Acta 1767 829–837 PubMed

Komenda J, Lupínková L, Kopecký J (2002) Absence of the psbH gene product destabilizes the photosystem II complex and bicarbonate binding on its acceptor side in Synechocystis PCC 6803. Eur J Biochem 269 610–619 PubMed

Komenda J, Reisinger V, Müller BCh, Dobáková M, Granvogl B, Eichacker LA (2004) Accumulation of the D2 protein is a key regulatory step for assembly of the photosystem II reaction center complex in Synechocystis PCC 6803. J Biol Chem 279 48620–48629 PubMed

Komenda J, Tichý M, Eichacker L (2005) The PsbH protein is associated with the inner antenna CP47 and facilitates D1 processing and incorporation into photosystem II in the cyanobacterium Synechocystis PCC 6803. Plant Cell Physiol 46 1477–1483 PubMed

Künstner P, Guardiola A, Takahashi Y, Rochaix JD (1995) A mutant strain of Chlamydomonas reinhardtii lacking chloroplast photosystem II psbI gene grows photoautotrophically. J Biol Chem 270 9651–9654 PubMed

Lagarde D, Beuf L, Vermaas W (2000) Increased production of zeaxanthin and other pigments by application of genetic engineering techniques to Synechocystis sp. strain PCC 6803. Appl Environ Microbiol 66 64–72 PubMed PMC

Loll B, Kern J, Saenger W, Zouni A, Biesiadka J (2005) Towards complete cofactor arrangement in the 3.0 Å resolution structure of photosystem II. Nature 438 1040–1044 PubMed

Müller B, Eichacker LA (1999) Assembly of the D1 precursor in monomeric photosystem II reaction center precomplexes precedes chlorophyll a-triggered accumulation of reaction center II in barley etioplasts. Plant Cell 11 2365–2377 PubMed PMC

Nanba O, Satoh K (1987) Isolation of a photosystem II reaction center consisting of D-1 and D-2 polypeptides and cytochrome b-559. Proc Natl Acad Sci USA 84 109–112 PubMed PMC

Nixon PJ, Trost JT, Diner BA (1992) Role of the carboxy terminus of polypeptide-D1 in the assembly of a functional water-oxidizing manganese cluster in photosystem-II of the cyanobacterium Synechocystis sp. PCC 6803: assembly requires a free carboxyl group at C-terminal position 344. Biochemistry 31 10859–10871 PubMed

Pakrasi HB, Williams JGK, Arntzen CJ (1988) Targeted mutagenesis of the psbE and psbF blocks photosynthetic electron transport: evidence for a functional role of cytochrome b-559 in photosystem II. EMBO J 7 325–332 PubMed PMC

Schägger H, von Jagow G (1991) Blue native electrophoresis for isolation of membrane protein complexes in enzymatically active form. Anal Biochem 199 223–231 PubMed

Schwenkert S, Umate P, Bosco CD, Volz S, Mlčochová L, Zoryan M, Eichacker LA, Ohad I, Herrman RG, Meurer J (2006) PsbI affects the stability, function and phosphorylation patterns of photosystem II assemblies in tobacco. J Biol Chem 281 34227–34238 PubMed

Takahashi M, Shiraishi T, Asada K (1988) COOH-terminal residues of D1 and the 44 kDa CPa-2 at spinach photosystem II core complex. FEBS Lett 240 6–8 PubMed

Tichý M, Lupínková L, Sicora C, Vass I, Kuviková S, Prášil O, Komenda J (2003) Synechocystis 6803 mutants expressing distinct forms of the photosystem II D1 protein from Synechococcus 7942: relationship between the psbA coding region and sensitivity to visible and UV-B radiation. Biochim Biophys Acta 1605 55–66 PubMed

Vermaas WFJ, Ikeuchi M, Inoue Y (1988) Protein composition of the photosystem II core complex in genetically engineered mutants of the cyanobacterium Synechocystis sp. PCC 6803. Photosynth Res 17 97–113 PubMed

Webber AN, Packham L, Chapman DJ, Barber J, Gray JC (1989) A fifth chloroplast-encoded polypeptide is present in the photosystem II reaction centre complex. FEBS Lett 242 259–262

Wellburn AR, Lichtenthaler K (1984) Formulae and programme to determine total carotenoids and chlorophyll a and b of leaf extracts in different solvents. In C Sybesma, ed, Advances in Photosynthesis Research. Martinus Nijjhoff, Dordrecht, The Netherlands, pp 10–12

Williams JGK (1988) Construction of specific mutations in PSII photosynthetic reaction center by genetic engineering methods in Synechocystis 6803. Methods Enzymol 167 766–778

Assembly of D1/D2 complexes of photosystem II: Binding of pigments and a network of auxiliary proteins

Tandem gene amplification restores photosystem II accumulation in cytochrome b559 mutants of cyanobacteria

Fast Diffusion of the Unassembled PetC1-GFP Protein in the Cyanobacterial Thylakoid Membrane

Purification of Protein-complexes from the Cyanobacterium Synechocystis sp. PCC 6803 Using FLAG-affinity Chromatography

Chlorophyll f synthesis by a super-rogue photosystem II complex

Ycf48 involved in the biogenesis of the oxygen-evolving photosystem II complex is a seven-bladed beta-propeller protein

The Ribosome-Bound Protein Pam68 Promotes Insertion of Chlorophyll into the CP47 Subunit of Photosystem II

High light acclimation of Chromera velia points to photoprotective NPQ

Discovery of a chlorophyll binding protein complex involved in the early steps of photosystem II assembly in Synechocystis

Psb28 protein is involved in the biogenesis of the photosystem II inner antenna CP47 (PsbB) in the cyanobacterium Synechocystis sp. PCC 6803

Importance of the cyanobacterial Gun4 protein for chlorophyll metabolism and assembly of photosynthetic complexes