8-isoprostane and leukotrienes in exhaled breath condensate in Czech subjects with silicosis
Language English Country Japan Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
18212471
DOI
10.2486/indhealth.45.766
PII: JST.JSTAGE/indhealth/45.766
Knihovny.cz E-resources
- MeSH
- Biomarkers analysis MeSH
- Time Factors MeSH
- Breath Tests MeSH
- Dinoprost analogs & derivatives analysis MeSH
- Mass Spectrometry MeSH
- Leukotrienes analysis MeSH
- Middle Aged MeSH
- Humans MeSH
- Respiratory Function Tests MeSH
- Aged MeSH
- Silicosis diagnosis physiopathology MeSH
- Chromatography, High Pressure Liquid MeSH
- Health Behavior MeSH
- Check Tag
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czech Republic MeSH
- Names of Substances
- 8-epi-prostaglandin F2alpha MeSH Browser
- Biomarkers MeSH
- Dinoprost MeSH
- Leukotrienes MeSH
Silicosis has progressive, but unpredictable development. There are no markers routinely available to measure the activity and prognosis of silicosis. 8-isoprostane, a marker of oxidative stress and leukotrienes B4, C4, D4, and E4 were measured in exhaled breath condensate in patients with silicosis. Sixty subjects were examined, with mean age 66.7+/-2.0 yr and mean exposure to silica 23.6+/-2.5 yr. The control group had 25 subjects, with mean age 64.7+/-4.8 yr. Exhaled breath was collected using the EcoScreen (Jaeger, Germany); 8-isoprostane and leukotrienes were analyzed by high-performance liquid chromatography/mass spectrometry. Several lung functions parameters were impaired in silicotics in comparisons with the controls; ESR, alpha1-antitrypsin and proteinuria were higher in the silicotics. Antineutrophilic cytoplasmic antibodies were more frequent in the silicotics (39%) than in the controls (4%), (p=0.0017). The mean level of 8-isoprostane in the patients with silicosis was 73.6+/-9.9 vs. 43+/-10 pg/ml (p=0.0001) in the controls. Silica exposure category (high, medium, none) was positively associated with the level of 8-isoprostane. In the patients with complicated silicosis, a high level of 8-isoprostane was found more frequently (p=0.0194). Leukotriene D4 was also increased in the silicotics (21.1+/-2.7 pg/ml) vs. the controls (14.7+/-2.7 pg/ml), (p=0.001). No significant effect of smoking or alcohol consumption on the markers examined was seen. This is the first study using exhaled breath condensate analysis in patients with silicosis.
References provided by Crossref.org
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Deep Airway Inflammation and Respiratory Disorders in Nanocomposite Workers
Markers of Oxidative Stress in the Exhaled Breath Condensate of Workers Handling Nanocomposites
