8-isoprostane and leukotrienes in exhaled breath condensate in Czech subjects with silicosis
Jazyk angličtina Země Japonsko Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
18212471
DOI
10.2486/indhealth.45.766
PII: JST.JSTAGE/indhealth/45.766
Knihovny.cz E-zdroje
- MeSH
- biologické markery analýza MeSH
- časové faktory MeSH
- dechové testy MeSH
- dinoprost analogy a deriváty analýza MeSH
- hmotnostní spektrometrie MeSH
- leukotrieny analýza MeSH
- lidé středního věku MeSH
- lidé MeSH
- respirační funkční testy MeSH
- senioři MeSH
- silikóza diagnóza patofyziologie MeSH
- vysokoúčinná kapalinová chromatografie MeSH
- zdravé chování MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
- Názvy látek
- 8-epi-prostaglandin F2alpha MeSH Prohlížeč
- biologické markery MeSH
- dinoprost MeSH
- leukotrieny MeSH
Silicosis has progressive, but unpredictable development. There are no markers routinely available to measure the activity and prognosis of silicosis. 8-isoprostane, a marker of oxidative stress and leukotrienes B4, C4, D4, and E4 were measured in exhaled breath condensate in patients with silicosis. Sixty subjects were examined, with mean age 66.7+/-2.0 yr and mean exposure to silica 23.6+/-2.5 yr. The control group had 25 subjects, with mean age 64.7+/-4.8 yr. Exhaled breath was collected using the EcoScreen (Jaeger, Germany); 8-isoprostane and leukotrienes were analyzed by high-performance liquid chromatography/mass spectrometry. Several lung functions parameters were impaired in silicotics in comparisons with the controls; ESR, alpha1-antitrypsin and proteinuria were higher in the silicotics. Antineutrophilic cytoplasmic antibodies were more frequent in the silicotics (39%) than in the controls (4%), (p=0.0017). The mean level of 8-isoprostane in the patients with silicosis was 73.6+/-9.9 vs. 43+/-10 pg/ml (p=0.0001) in the controls. Silica exposure category (high, medium, none) was positively associated with the level of 8-isoprostane. In the patients with complicated silicosis, a high level of 8-isoprostane was found more frequently (p=0.0194). Leukotriene D4 was also increased in the silicotics (21.1+/-2.7 pg/ml) vs. the controls (14.7+/-2.7 pg/ml), (p=0.001). No significant effect of smoking or alcohol consumption on the markers examined was seen. This is the first study using exhaled breath condensate analysis in patients with silicosis.
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