Novel approach to fast determination of multiple pesticide residues using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)
Language English Country Great Britain, England Media print
Document type Journal Article, Research Support, Non-U.S. Gov't, Validation Study
PubMed
18348044
DOI
10.1080/02652030701570156
PII: 790131527
Knihovny.cz E-resources
- MeSH
- Time Factors MeSH
- European Union MeSH
- Calibration MeSH
- Infant MeSH
- Humans MeSH
- Malus chemistry MeSH
- Maximum Allowable Concentration MeSH
- Infant Food standards MeSH
- Reference Standards MeSH
- Reproducibility of Results MeSH
- Pesticide Residues analysis MeSH
- Quality Control MeSH
- Tandem Mass Spectrometry methods MeSH
- Chromatography, High Pressure Liquid methods MeSH
- Check Tag
- Infant MeSH
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Validation Study MeSH
- Names of Substances
- Pesticide Residues MeSH
A rapid, high-throughput method employing ultra-performance liquid chromatography with tandem quadrupole mass spectrometry (UPLC-MS/MS) was developed and optimized for simultaneous quantification and confirmation of 64 pesticide residues and their toxic metabolites in fruit extracts prepared by a buffered QuEChERS procedure. The total time required for UPLC-MS/MS analysis was 8 min plus 2 min for re-equilibration to the initial UPLC conditions. Performance characteristics were determined for apple extracts spiked at 10 microg kg(-1). The repeatability of measurements expressed as relative standard deviations was in the range 1.5-13% at this level for most analytes. Thanks to very low limits of quantification (<10 microg kg(-1)for the majority of pesticides), an optimized method allows for the reliable control of not only common maximum residue limits (MRLs) set by European Union regulation for various pesticides/fruit combinations, but also of a uniform MRL of 10 microg kg(-1)endorsed for baby food.
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