Detection of methicillin and mupirocin resistance in staphylococcal hospital isolates with a touchdown multiplex polymerase chain reaction
Language English Country United States Media print-electronic
Document type Evaluation Study, Journal Article
- MeSH
- Anti-Bacterial Agents pharmacology MeSH
- Bacterial Proteins genetics metabolism MeSH
- DNA, Bacterial genetics MeSH
- Humans MeSH
- Methicillin pharmacology MeSH
- Drug Resistance, Multiple, Bacterial * MeSH
- Mupirocin pharmacology MeSH
- Hospitals MeSH
- Polymerase Chain Reaction methods MeSH
- Methicillin Resistance MeSH
- RNA, Ribosomal, 16S genetics MeSH
- Sensitivity and Specificity MeSH
- Staphylococcal Infections microbiology MeSH
- Staphylococcus drug effects genetics isolation & purification MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Names of Substances
- Anti-Bacterial Agents MeSH
- Bacterial Proteins MeSH
- DNA, Bacterial MeSH
- Methicillin MeSH
- Mupirocin MeSH
- RNA, Ribosomal, 16S MeSH
Staphylococcal hospital isolates (n = 166) were tested in a touchdown multiplex-polymerase chain reaction assay for the identification of methicillin and mupirocin resistance and discrimination of S. aureus (femA gene) from coagulase negative staphylococci and other bacteria. All isolates harbored the 16SrDNA (Staphylococcus genus specific internal control) gene, and 130 (78 %) the mecA (methicillin resistance) gene. Fifty-seven (44 %) of these were determined as methicillin-resistant S. aureus, while the remaining 73 (56 %) were methicillin-resistant coagulase-negative staphylococci. Seventy-five (45 %) isolates harbored the ileS-2 (high-level mupirocin resistance) gene and were determined as mupirocin-resistant. This assay represents a simple, rapid, reliable approach for the detection and discrimination of methicillin-and mupirocin-resistant staphylococci.
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