Micromodification of virus-neutralisation assay with vital staining in 96-well plate and its use in diagnostics of Tahyna virus infections
Language English Country Czech Republic Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
18767378
Knihovny.cz E-resources
- MeSH
- Coloring Agents MeSH
- Child MeSH
- Adult MeSH
- Encephalitis, California diagnosis epidemiology MeSH
- Cells, Cultured MeSH
- Middle Aged MeSH
- Humans MeSH
- Adolescent MeSH
- Neutralization Tests * MeSH
- Neutral Red MeSH
- Child, Preschool MeSH
- Antibodies, Viral analysis MeSH
- Aged MeSH
- Seroepidemiologic Studies MeSH
- Encephalitis Virus, California immunology MeSH
- Check Tag
- Child MeSH
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Adolescent MeSH
- Male MeSH
- Child, Preschool MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czech Republic epidemiology MeSH
- Names of Substances
- Coloring Agents MeSH
- Neutral Red MeSH
- Antibodies, Viral MeSH
Virus neutralization test (VNT) is considered to be the gold standard for arbovirus serology because of its high specificity and sensitivity. Its micromodification in 96-well plate with vital staining of cell cultures was developed in the National Reference Laboratory (NRL) for Arboviruses of the Czech Republic and is used for the detection of specific antibodies against various viruses, mainly arboviruses. The test procedure is described for the Tahyna virus micromodified neutralization assay in 96-well plate. Results of an anti-Tahyna antibody survey conducted among the population of northern Moravia using the VNT assay are presented. The overall anti-Tahyna seroprevalence among 1001 tested persons was 3.80%. The highest positivity rate was found in persons aged over 59 years (17.53%) comparing to children with the seroprevalence rates of 0.00% and 0.56% in the age groups 0-5 years and 6-14 years, respectively.
History of Arbovirus Research in the Czech Republic
