Comparative analysis of Salmonella enterica serovar Enteritidis mutants with a vaccine potential
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem
PubMed
19577637
DOI
10.1016/j.vaccine.2009.06.060
PII: S0264-410X(09)00923-2
Knihovny.cz E-zdroje
- MeSH
- atenuované vakcíny imunologie MeSH
- bakteriální geny MeSH
- delece genu MeSH
- myši inbrední BALB C MeSH
- myši MeSH
- polymyxiny imunologie MeSH
- reprodukovatelnost výsledků MeSH
- Salmonella enteritidis genetika imunologie patogenita MeSH
- salmonelová infekce u zvířat imunologie prevence a kontrola MeSH
- salmonelové vakcíny imunologie MeSH
- virulence MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- atenuované vakcíny MeSH
- polymyxiny MeSH
- salmonelové vakcíny MeSH
If any new live Salmonella vaccine is introduced in the future, it is quite probable that detailed characterisation of its attenuation will be required. In this study we therefore compared 34 isogenic mutants of S. Enteritidis in aroA, aroD, galE, ssrA, sseA, phoP, rpoS, ompR, htrA, clpP, lon, rfaL, rfaG, rfaC, hfq, sodCI, hilA, sipA, avrA, sopB, sopA, sopE, sifA, shdA, fliC, fur, relA, spoT, rel-spoT, misL, rmbA, STM4258, STM4259 and spvBC genes for their resistance to stresses likely to be expected in the host and for their virulence and immunogenicity in Balb/C mice. We found that the cold and bile resistances essentially did not correlate with the resistances to other stress factors. Resistance to acid pH, heat, polymyxin and serum correlated with each other and also with the attenuation. When the residual virulence and immunogenicity were both considered, mutants in htrA, ompR, aroA, aroD and lon performed the best in mice. Furthermore, when a detailed comparison of polymyxin and serum sensitive mutants was performed, the serum sensitive mutants were more immunogenic.
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