The prevalence of Salmonella enterica serovar Enteritidis is gradually decreasing in poultry flocks in the EU, which may result in the demand for a vaccine that allows for the differentiation of vaccinated flocks from those infected by wild-type S. Enteritidis. In this study, we therefore constructed a (Salmonella Pathogenicity Island 1) SPI1-lon mutant with or without fliC encoding for S. Enteritidis flagellin. The combination of SPI1-lon mutations resulted in attenuated but immunogenic mutant suitable for oral vaccination of poultry. In addition, the vaccination of chickens with the SPI1-lon-fliC mutant enabled the serological differentiation of vaccinated and infected chickens. The absence of fliC therefore did not affect the immunogenicity of the vaccine strain and allowed for serological differentiation of the vaccinated chickens. The SPI1-lon-fliC mutant is therefore a suitable marker vaccine strain for oral vaccination of poultry.

Veterinary Research Institute Brno Czech Republic

Zobrazit více v PubMed

Berndt A, Wilhelm A, Jugert C, Pieper J, Sachse K, et al. (2007) Chicken cecum immune response to PubMed PMC

Matulova M, Rajova J, Vlasatikova L, Volf J, Stepanova H, et al. (2012) Characterization of chicken spleen transcriptome after infection with PubMed PMC

Matulova M, Varmuzova K, Sisak F, Havlickova H, Babak V, et al. (2013) Chicken innate immune response to oral infection with PubMed PMC

Beal RK, Wigley P, Powers C, Hulme SD, Barrow PA, et al. (2004) Age at primary infection with PubMed

Matulova M, Havlickova H, Sisak F, Rychlik I (2012) Vaccination of chickens with Salmonella Pathogenicity Island (SPI) 1 and SPI2 defective mutants of PubMed

Lahuerta A, Westrell T, Takkinen J, Boelaert F, Rizzi V, et al.. (2011) Zoonoses in the European Union: origin, distribution and dynamics - the EFSA-ECDC summary report 2009. Euro Surveill 16: pii: 19832. PubMed

Adriaensen C, De Greve H, Tian JQ, De Craeye S, Gubbels E, et al. (2007) A live PubMed PMC

Selke M, Meens J, Springer S, Frank R, Gerlach GF (2007) Immunization of pigs to prevent disease in humans: construction and protective efficacy of a PubMed PMC

Methner U, Barrow PA, Berndt A, Rychlik I (2011) PubMed

Gewirtz AT, Navas TA, Lyons S, Godowski PJ, Madara JL (2001) Cutting edge: bacterial flagellin activates basolaterally expressed TLR5 to induce epithelial proinflammatory gene expression. J Immunol 167: 1882–5. PubMed

Hayashi F, Smith KD, Ozinsky A, Hawn TR, Yi EC, et al. (2001) The innate immune response to bacterial flagellin is mediated by Toll-like receptor 5. Nature 410: 1099–103. PubMed

Iqbal M, Philbin VJ, Withanage GS, Wigley P, Beal RK, et al. (2005) Identification and functional characterization of chicken toll-like receptor 5 reveals a fundamental role in the biology of infection with PubMed PMC

Kremer CJ, O’Meara KM, Layton SL, Hargis BM, Cole K (2011) Evaluation of recombinant PubMed

McNeilly TN, Naylor SW, Mahajan A, Mitchell MC, McAteer S, et al. (2008) PubMed PMC

Kodama C, Matsui H (2004) PubMed PMC

Jones MA, Wigley P, Page KL, Hulme SD, Barrow PA (2001) PubMed PMC

Karasova D, Sebkova A, Vrbas V, Havlickova H, Sisak F, et al. (2009) Comparative analysis of PubMed

Murray RA, Lee CA (2000) Invasion genes are not required for PubMed PMC

Dieye Y, Ameiss K, Mellata M, Curtiss R III (2009) The Salmonella Pathogenicity Island (SPI) 1 contributes more than SPI2 to the colonization of the chicken by Salmonella enterica serovar Typhimurium. BMC Microbiol 9: 3. PubMed PMC

Rychlik I, Karasova D, Sebkova A, Volf J, Sisak F, et al. (2009) Virulence potential of five major pathogenicity islands (SPI-1 to SPI-5) of PubMed PMC

Monack DM, Raupach B, Hromockyj AE, Falkow S (1996) PubMed PMC

Pavlova B, Volf J, Ondrackova P, Matiasovic J, Stepanova H, et al. (2011) SPI-1-encoded type III secretion system of PubMed PMC

Pullinger GD, Paulin SM, Charleston B, Watson PR, Bowen AJ, et al. (2007) Systemic translocation of PubMed PMC

Gottesman S, Trisler P, Torres-Cabassa A (1985) Regulation of capsular polysaccharide synthesis in PubMed PMC

Takaya A, Tomoyasu T, Tokumitsu A, Morioka M, Yamamoto T (2002) The ATP-dependent lon protease of PubMed PMC

Leyman B, Boyen F, Van Parys A, Verbrugghe E, Haesebrouck F, et al. (2012) Tackling the issue of environmental survival of live PubMed

Matsuda K, Chaudhari AA, Kim SW, Lee KM, Lee JH (2010) Physiology, pathogenicity and immunogenicity of PubMed PMC

Nandre RM, Chaudhari AA, Matsuda K, Lee JH (2011) Immunogenicity of a PubMed

Slattery A, Victorsen AH, Brown A, Hillman K, Phillips GJ (2013) Isolation of Highly Persistent Mutants of PubMed PMC

Garcia-Calderon CB, Casadesus J, Ramos-Morales F (2007) Rcs and PhoPQ regulatory overlap in the control of PubMed PMC

Wang Q, Zhao Y, McClelland M, Harshey RM (2007) The RcsCDB signaling system and swarming motility in PubMed PMC

Andersen-Nissen E, Smith KD, Strobe KL, Barrett SL, Cookson BT, et al. (2005) Evasion of Toll-like receptor 5 by flagellated bacteria. Proc Natl Acad Sci U S A 102: 9247–52. PubMed PMC

Karasova D, Sebkova A, Havlickova H, Sisak F, Volf J, et al. (2010) Influence of 5 major PubMed PMC

Datsenko KA, Wanner BL (2000) One-step inactivation of chromosomal genes in PubMed PMC

Interleukin 4 inducible 1 gene (IL4I1) is induced in chicken phagocytes by Salmonella Enteritidis infection

Immune protection of chickens conferred by a vaccine consisting of attenuated strains of Salmonella Enteritidis, Typhimurium and Infantis

Gene expression in the chicken caecum in response to infections with non-typhoid Salmonella