Vaccination of chickens with Salmonella Pathogenicity Island (SPI) 1 and SPI2 defective mutants of Salmonella enterica serovar Enteritidis
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
22300724
DOI
10.1016/j.vaccine.2012.01.050
PII: S0264-410X(12)00088-6
Knihovny.cz E-resources
- MeSH
- Administration, Oral MeSH
- Vaccines, Attenuated administration & dosage genetics immunology MeSH
- Bacterial Load MeSH
- Cytokines metabolism MeSH
- Genomic Islands * MeSH
- Chickens MeSH
- Leukocytes, Mononuclear immunology MeSH
- Poultry Diseases immunology prevention & control MeSH
- Antibodies, Bacterial blood MeSH
- Salmonella enteritidis immunology pathogenicity MeSH
- Salmonella Infections, Animal immunology microbiology pathology prevention & control MeSH
- Salmonella Vaccines administration & dosage genetics immunology MeSH
- Immunization, Secondary methods MeSH
- Spleen pathology MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Vaccines, Attenuated MeSH
- Cytokines MeSH
- Antibodies, Bacterial MeSH
- Salmonella Vaccines MeSH
In this study we were interested in the vaccine potential of two attenuated mutants of Salmonella enterica serovar Enteritidis for poultry. The first mutant was attenuated by the removal of the whole Salmonella Pathogenicity Island 1 (SPI1) and the second mutant was devoid of the whole SPI2. These 2 mutants were used for oral vaccination of 2 chicken lines; Lohmann Brown and ISA Brown. Chickens were vaccinated orally on day 1 of life, revaccinated on day 21 and challenged on day 42. The challenge was performed either orally or intravenously. Despite a slightly different response between the two chicken lines, both the mutants gave protection to poultry against S. Enteritidis challenge as documented by findings such as the bacterial counts in tissues, spleen weight, antibody production and cytokine response (namely IL-17 and IL-22). When the 2 mutants were compared, vaccination with the SPI1 mutant proved to be more effective in the protection of poultry against S. Enteritidis challenge than the vaccination with the SPI2 mutant. On the other hand, vaccination with the SPI2 mutant stimulated a slightly higher antibody production and such a mutant might therefore be a better choice if Salmonella is used as a vector for the delivery of heterologous antigens with a desired stimulation of the humoral part of the immune system.
References provided by Crossref.org
Gene expression in the chicken caecum in response to infections with non-typhoid Salmonella
Chicken innate immune response to oral infection with Salmonella enterica serovar Enteritidis
