Comparative analysis of Salmonella enterica serovar Enteritidis mutants with a vaccine potential
Language English Country Netherlands Media print-electronic
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
19577637
DOI
10.1016/j.vaccine.2009.06.060
PII: S0264-410X(09)00923-2
Knihovny.cz E-resources
- MeSH
- Vaccines, Attenuated immunology MeSH
- Genes, Bacterial MeSH
- Gene Deletion MeSH
- Mice, Inbred BALB C MeSH
- Mice MeSH
- Polymyxins immunology MeSH
- Reproducibility of Results MeSH
- Salmonella enteritidis genetics immunology pathogenicity MeSH
- Salmonella Infections, Animal immunology prevention & control MeSH
- Salmonella Vaccines immunology MeSH
- Virulence MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Vaccines, Attenuated MeSH
- Polymyxins MeSH
- Salmonella Vaccines MeSH
If any new live Salmonella vaccine is introduced in the future, it is quite probable that detailed characterisation of its attenuation will be required. In this study we therefore compared 34 isogenic mutants of S. Enteritidis in aroA, aroD, galE, ssrA, sseA, phoP, rpoS, ompR, htrA, clpP, lon, rfaL, rfaG, rfaC, hfq, sodCI, hilA, sipA, avrA, sopB, sopA, sopE, sifA, shdA, fliC, fur, relA, spoT, rel-spoT, misL, rmbA, STM4258, STM4259 and spvBC genes for their resistance to stresses likely to be expected in the host and for their virulence and immunogenicity in Balb/C mice. We found that the cold and bile resistances essentially did not correlate with the resistances to other stress factors. Resistance to acid pH, heat, polymyxin and serum correlated with each other and also with the attenuation. When the residual virulence and immunogenicity were both considered, mutants in htrA, ompR, aroA, aroD and lon performed the best in mice. Furthermore, when a detailed comparison of polymyxin and serum sensitive mutants was performed, the serum sensitive mutants were more immunogenic.
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