Genetic polymorphisms influence the susceptibility of men to sperm DNA damage associated with exposure to air pollution
Language English Country Netherlands Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
19800896
DOI
10.1016/j.mrfmmm.2009.09.010
PII: S0027-5107(09)00290-5
Knihovny.cz E-resources
- MeSH
- Chromatin genetics MeSH
- Cytochrome P-450 CYP1A1 genetics metabolism MeSH
- Adult MeSH
- DNA Repair Enzymes genetics metabolism MeSH
- DNA Fragmentation drug effects MeSH
- Genotype MeSH
- Glutathione Transferase genetics metabolism MeSH
- Cotinine urine MeSH
- Smoking MeSH
- Folic Acid metabolism MeSH
- Air Pollutants, Occupational adverse effects MeSH
- Humans MeSH
- Police MeSH
- Polymerase Chain Reaction MeSH
- Polymorphism, Restriction Fragment Length MeSH
- Polymorphism, Genetic * MeSH
- DNA Damage genetics MeSH
- Spermatozoa drug effects MeSH
- Xeroderma Pigmentosum Group D Protein genetics metabolism MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Male MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Chromatin MeSH
- Cytochrome P-450 CYP1A1 MeSH
- DNA Repair Enzymes MeSH
- ERCC2 protein, human MeSH Browser
- glutathione S-transferase M1 MeSH Browser
- Glutathione Transferase MeSH
- Cotinine MeSH
- Folic Acid MeSH
- Air Pollutants, Occupational MeSH
- Xeroderma Pigmentosum Group D Protein MeSH
The purpose of the present study was to investigate the impact of carcinogenic polycyclic aromatic hydrocarbons and volatile organic compounds on sperm quality in a group of city policemen in Prague during a period of increased concentrations of ambient air-pollutants (winter season) compared to a period of low exposure (spring). Polymorphisms in metabolic genes (CYP1A1, EPHX1, GSTM1, GSTP1, GSTT1), folic acid metabolism genes (MTR, MTHFR) and DNA repair genes (XRCC1, XPD6, XPD23, hOGG1) were evaluated in these men as potential modifiers of associations between air pollution exposure and changes in sperm quality. The study population was a group of 47 policemen working in the center of the city. Seasonal differences in exposure were verified by ambient and personal monitoring. Markers of sperm injury included semen volume, sperm concentration, sperm morphology, sperm motility, and sperm DNA damage measured with the sperm chromatin structure assay The sperm chromatin structure assay (SCSA) includes a measure of DNA damage called DNA Fragmentation Index (DFI). The % of cells with detectable DFI (detDFI) by this assay includes sperm with either medium or high DNA damage; the term hDFI is used to define the % of sperm with only high DNA damage. The assay also detects immature sperm defined by high density staining (HDS). No significant differences were found in any of the standard semen parameters between the sampling periods except for vitality of sperms. Both DFI and HDS were significantly higher in winter than in spring samples for all men and for non-smokers. At the bivariate level, significant associations between hDFI or detDFI and polymorphisms of the repair genes XRCC1, XPD6 and XPD23 were observed. In multivariate models, polymorphisms of the genes XPD6, XPD23 and CYP1A1MspI were associated with hDFI and HDS. Moreover, HDS was significantly associated with polymorphisms in GSTM1 gene.
References provided by Crossref.org
Impact of Air Pollution on the Health of the Population in Parts of the Czech Republic
