Oxidation changes physical properties of phospholipid bilayers: fluorescence spectroscopy and molecular simulations
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
20387820
DOI
10.1021/la100657a
Knihovny.cz E-resources
- MeSH
- Cell Membrane chemistry MeSH
- Diffusion MeSH
- Spectrometry, Fluorescence MeSH
- Phosphatidylcholines chemistry MeSH
- Phospholipid Ethers chemistry MeSH
- Phospholipids chemistry MeSH
- Physical Phenomena * MeSH
- Lipid Bilayers chemistry MeSH
- Oxidation-Reduction MeSH
- Solvents chemistry MeSH
- Molecular Dynamics Simulation * MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- 1-palmitoyl-2-(5-oxovaleroyl)-sn-glycero-3-phosphorylcholine MeSH Browser
- 1-palmitoyl-2-oleoylphosphatidylcholine MeSH Browser
- Phosphatidylcholines MeSH
- Phospholipid Ethers MeSH
- Phospholipids MeSH
- Lipid Bilayers MeSH
- Solvents MeSH
Physical properties of oxidized phospholipid (OxPL) membranes consisting of binary mixtures of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and 10 mol % of one of two OxPLs, 1-palmitoyl-2-glutaryl-sn-glycero-3-phosphocholine (PGPC) or 1-palmitoyl-2-(5'-oxo-valeroyl)-sn-glycero-3-phosphocholine (POVPC), were investigated experimentally and computationally. Fluorescence solvent relaxation (SR) and fluorescence correlation spectroscopy z-scan (FCS z-scan) show increased headgroup hydration and mobility, and faster lateral diffusion in POPC membrane upon addition of OxPLs. The magnitudes of both effects are distinct for each of the two OxPLs. Molecular dynamics simulations corroborate the experimental findings, providing at the same time a detailed molecular interpretation in terms of changes in bilayer structure and phospholipid orientation.
References provided by Crossref.org
What Does Time-Dependent Fluorescence Shift (TDFS) in Biomembranes (and Proteins) Report on?
Time-resolved fluorescence in lipid bilayers: selected applications and advantages over steady state
