Neonatal colonization of mice with Lactobacillus plantarum producing the aeroallergen Bet v 1 biases towards Th1 and T-regulatory responses upon systemic sensitization
Language English Country Denmark Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Allergens administration & dosage genetics immunology MeSH
- Antigens, Plant genetics immunology MeSH
- Betula genetics immunology MeSH
- Hypersensitivity, Immediate MeSH
- Cytokines biosynthesis immunology MeSH
- Forkhead Transcription Factors genetics metabolism MeSH
- Immunization * MeSH
- Immunoglobulin Isotypes blood immunology MeSH
- Lactobacillus plantarum genetics immunology MeSH
- Mice, Inbred BALB C MeSH
- Mice MeSH
- Animals, Newborn MeSH
- Pollen genetics immunology MeSH
- T-Lymphocytes, Regulatory immunology MeSH
- Spleen cytology immunology MeSH
- Th1 Cells immunology MeSH
- Th2 Cells immunology MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Allergens MeSH
- Antigens, Plant MeSH
- Cytokines MeSH
- Forkhead Transcription Factors MeSH
- Foxp3 protein, mouse MeSH Browser
- Immunoglobulin Isotypes MeSH
BACKGROUND: The use of recombinant lactic acid bacteria (LAB) as vehicles for mucosal delivery of recombinant allergens is an attractive concept for antigen-defined allergy prevention/treatment. Interventions with LAB are of increasing interest early in life when immune programming is initiated. Here, we investigated the effect of neonatal colonization with a recombinant LAB producing the major birch pollen allergen Bet v 1 in a murine model of type I allergy. METHODS: We constructed a recombinant Lactobacillus (L.) plantarum NCIMB8826 strain constitutively producing Bet v 1 to be used for natural mother-to-offspring mono-colonization of germ-free BALB/c mice. Allergen-specific immunomodulatory effects of the colonization on humoral and cellular immune responses were investigated prior and after sensitization to Bet v 1. RESULTS: Mono-colonization with the Bet v 1 producing L. plantarum induced a Th1-biased immune response at the cellular level, evident in IFN-γ production of splenocytes upon stimulation with Bet v 1. After sensitization with Bet v 1 these mice displayed suppressed IL-4 and IL-5 production in spleen and mesenteric lymph node cell cultures as well as decreased allergen-specific antibody responses (IgG1, IgG2a, and IgE) in sera. This suppression was associated with a significant up-regulation of the regulatory marker Foxp3 at the mRNA level in the spleen cells. CONCLUSION: Intervention at birth with a live recombinant L. plantarum producing a clinically relevant allergen reduces experimental allergy and might therefore become an effective strategy for early intervention against the onset of allergic diseases.
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