Transmitted-light coherence-controlled holographic microscope (CCHM) based on an off-axis achromatic interferometer allows us to use light sources of arbitrary degree of temporal and spatial coherence. Besides the conventional DHM modes such as quantitative phase contrast imaging and numerical 3D holographic reconstruction it provides high quality (speckle-free) imaging, improved lateral resolution and optical sectioning by coherence gating. Optical setup parameters and their limits for a technical realization are derived and described in detail. To demonstrate the optical sectioning property of the microscope a model sample uncovered and then covered with a diffuser was observed using a low-coherence light source.

Institute of Physical Engineering Faculty of Mechanical Engineering Brno University of Technology Technicka 2 616 69 Brno Czech Republic

References provided by Crossref.org

Two-dimensional quantitative near-field phase imaging using square and hexagonal interference devices

Automated interpretation of time-lapse quantitative phase image by machine learning to study cellular dynamics during epithelial-mesenchymal transition

Quantitative Phase Imaging of Spreading Fibroblasts Identifies the Role of Focal Adhesion Kinase in the Stabilization of the Cell Rear

The Quantitative-Phase Dynamics of Apoptosis and Lytic Cell Death

Cell segmentation methods for label-free contrast microscopy: review and comprehensive comparison

Geometric-Phase Microscopy for Quantitative Phase Imaging of Isotropic, Birefringent and Space-Variant Polarization Samples

Holography microscopy as an artifact-free alternative to phase-contrast

Oxidative Stress Resistance in Metastatic Prostate Cancer: Renewal by Self-Eating

Automated alignment method for coherence-controlled holographic microscope

Multimodal holographic microscopy: distinction between apoptosis and oncosis

Digital holographic microscopy in human sperm imaging