Cytotypes of Kirk's dik-dik (Madoqua kirkii,Bovidae) show multiple tandem fusions
Language English Country Switzerland Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
21124018
DOI
10.1159/000322483
PII: 000322483
Knihovny.cz E-resources
- MeSH
- Antelopes genetics MeSH
- Centromere genetics MeSH
- In Situ Hybridization, Fluorescence methods MeSH
- Karyotyping methods MeSH
- Chromosome Painting methods MeSH
- Evolution, Molecular * MeSH
- Chromosome Banding methods MeSH
- DNA, Satellite genetics MeSH
- Chromosomes, Mammalian genetics MeSH
- Base Sequence MeSH
- Telomere genetics MeSH
- Animals MeSH
- Check Tag
- Male MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA, Satellite MeSH
Madoqua kirkii, a miniature African antelope, is noted for extensive chromosomal variation that has been categorized in four distinct cytotypes (A-D). In this investigation, we analyzed the A cytotype (2n = 46, FN = 48) using a suite of molecular cytogenetic approaches that entailed (i) whole chromosome and subchromosomal painting by fluorescence in situ hybridization (FISH), (ii) the study of Madoqua centromeric-specific DNA derived from pooled DNA obtained from the centromeric regions of the acrocentric chromosomes, and (iii) DNA from the telomere:centromere junctions of tandemly fused chromosomes. DNA from these sources was used to probe for the persistence of interstitial satellite DNA and residual centromeric sequences in the tandem and centric fusion junctions by PCR and FISH. The analyses show centromeric sequences at two of the six tandem fusion junctions. These data, and those of hybrid specimens (A × B cytotypes) in conjunction with published information permitted an interpretation of the probable sequence of chromosomal rearrangements among the M. kirkii cytotypes. We discuss the findings in the context of chromosomal evolution in these antelopes, and the implications that these hold for ex-situ breeding programs of the species.
References provided by Crossref.org
X Chromosome-Specific Repeats in Non-Domestic Bovidae
Sequence Analysis and FISH Mapping of Four Satellite DNA Families among Cervidae
Karyotype relationships among selected deer species and cattle revealed by bovine FISH probes
Meiotic behaviour of evolutionary sex-autosome translocations in Bovidae
Tribe-specific satellite DNA in non-domestic Bovidae
