The evaluation of survival and proliferation of lymphocytes in autologous mixed leukocyte reaction with dendritic cells. The comparison of incorporation of (3)H-thymidine and differential gating method
Language English Country Netherlands Media print-electronic
Document type Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
21802072
DOI
10.1016/j.cellimm.2011.06.006
PII: S0008-8749(11)00144-4
Knihovny.cz E-resources
- MeSH
- Antigens, CD immunology metabolism MeSH
- CD4-Positive T-Lymphocytes immunology metabolism pathology MeSH
- CD8-Positive T-Lymphocytes immunology metabolism pathology MeSH
- Leukemia, Lymphocytic, Chronic, B-Cell immunology pathology MeSH
- Dendritic Cells immunology pathology MeSH
- Cells, Cultured MeSH
- Middle Aged MeSH
- Humans MeSH
- Lipopolysaccharide Receptors immunology metabolism MeSH
- Lymphocytes immunology metabolism pathology MeSH
- Monocytes immunology metabolism pathology MeSH
- Cell Proliferation * MeSH
- Flow Cytometry methods MeSH
- Receptors, Transferrin immunology metabolism MeSH
- Reproducibility of Results MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Lymphocyte Culture Test, Mixed methods MeSH
- Thymidine metabolism MeSH
- Tritium MeSH
- Cell Survival immunology MeSH
- Check Tag
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Aged, 80 and over MeSH
- Aged MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Antigens, CD MeSH
- CD71 antigen MeSH Browser
- Lipopolysaccharide Receptors MeSH
- Receptors, Transferrin MeSH
- Thymidine MeSH
- Tritium MeSH
Dendritic cells (DCs) play the key role in T-lymphocyte proliferation and induction of antitumour response. The mixed leukocyte reaction (MLR) of T-lymphocytes and DCs is essential instrument for immunological mechanisms studies. Conventionally used method for determination of T-lymphocytes proliferation, (3)H-thymidine incorporation, provides only general information. The method of flow cytometry and differential gating seems to be more suitable for quantitative and qualitative analysis of T-lymphocyte proliferation. It is based on time limited acquisition of events and on its distribution according to forward and side scatter values. We decided to compare these two methods and determine mutual correlation and compatibility. Eleven patients were studied and in all cases DCs promoted the survival and proliferation of both CD4 and CD8 lymphocytes. Both methods retained consistency with regard to survival and proliferation of CD4/CD8 lymphocytes. However, the correlation of these methods was not convincing. Therefore, both these methods might be used for evaluation of MLR, but each of them gives specific and complementary information.
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