Hypomethylating drugs efficiently decrease cytosine methylation in telomeric DNA and activate telomerase without affecting telomere lengths in tobacco cells
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- adenin analogy a deriváty farmakologie MeSH
- aktivace enzymů účinky léků MeSH
- cytidin analogy a deriváty farmakologie MeSH
- DNA rostlinná chemie účinky léků MeSH
- epigeneze genetická MeSH
- genetická transkripce účinky léků MeSH
- kultivované buňky MeSH
- metylace DNA účinky léků MeSH
- nukleozomy účinky léků fyziologie MeSH
- rostlinné proteiny genetika metabolismus MeSH
- tabák cytologie účinky léků genetika metabolismus MeSH
- telomerasa metabolismus MeSH
- telomery chemie účinky léků metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- 9-(2,3-dihydroxypropyl)adenine MeSH Prohlížeč
- adenin MeSH
- cytidin MeSH
- DNA rostlinná MeSH
- nukleozomy MeSH
- pyrimidin-2-one beta-ribofuranoside MeSH Prohlížeč
- rostlinné proteiny MeSH
- telomerasa MeSH
Telomere homeostasis is regulated at multiple levels, including the local chromatin structure of telomeres and subtelomeres. Recent reports demonstrated that a decrease in repressive chromatin marks, such as levels of cytosine methylation in subtelomeric regions, results in telomere elongation in mouse cells. Here we show that a considerable fraction of cytosines is methylated not only in subtelomeric, but also in telomeric DNA of tobacco BY-2 cells. Drug-induced hypomethylation (demonstrated at subtelomeric, telomeric, and global DNA levels) results in activation of telomerase. However, in contrast to mouse cells, the decrease in 5-methylcytosine levels and upregulation of telomerase do not result in any changes of telomere lengths. These results demonstrate the involvement of epigenetic mechanisms in the multilevel process of regulation of telomerase activity in plant cells and, at the same time, they indicate that changes in telomerase activity can be overridden by other factors governing telomere length stability.
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Telomeres in Plants and Humans: Not So Different, Not So Similar
Telomere- and Telomerase-Associated Proteins and Their Functions in the Plant Cell
Chromatin features of plant telomeric sequences at terminal vs. internal positions
Compromised telomere maintenance in hypomethylated Arabidopsis thaliana plants
Three TERT genes in Nicotiana tabacum