Surface plasmon resonance biosensor for the detection of VEGFR-1--a protein marker of myelodysplastic syndromes
Language English Country Germany Media print-electronic
Document type Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Biomarkers blood MeSH
- Biosensing Techniques methods MeSH
- Humans MeSH
- Myelodysplastic Syndromes blood diagnosis metabolism MeSH
- Surface Plasmon Resonance methods MeSH
- Vascular Endothelial Growth Factor Receptor-1 blood metabolism MeSH
- Vascular Endothelial Growth Factor A blood metabolism MeSH
- Protein Binding MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Biomarkers MeSH
- Vascular Endothelial Growth Factor Receptor-1 MeSH
- Vascular Endothelial Growth Factor A MeSH
The surface plasmon resonance (SPR) biosensor system with dispersionless microfluidics for the direct and label-free detection of a soluble vascular endothelial growth factor receptor (sVEGFR-1) is described. The detection approach takes advantage of an affinity interaction between sVEGFR-1 and its ligand, vascular endothelial growth factor (VEGF-A), which is covalently immobilized on the surface of the SPR sensor. The ability of the immobilized VEGF-A to specifically bind the sVEGFR-1 receptor is demonstrated in a buffer. The detection of sVEGFR-1 in 2% human blood plasma is carried out by using the sequential injection approach. The detection limit of 25 ng/mL is achieved. In addition, we demonstrate that the functional surface of the sensor can be regenerated for repeated use.
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