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Three TERT genes in Nicotiana tabacum

. 2012 May ; 20 (4) : 381-94. [epub] 20120428

Language English Country Netherlands Media print-electronic

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/pmid22543812

Telomerase is essential for proper functioning of telomeres in eukaryotes. We cloned and characterised genes for the protein subunit of telomerase (TERT) in the allotetraploid Nicotiana tabacum (tobacco) and its diploid progenitor species Nicotiana sylvestris and Nicotiana tomentosiformis with the aim of determining if allopolyploidy (hybridisation and genome duplication) influences TERT activity and divergence. Two of the three sequence variants present in the tobacco genome (NtTERT-C/s and NtTERT-D) revealed similarity to two sequence variants found in N. sylvestris and another variant (NtTERT-C/t) was similar to TERT of N. tomentosiformis. Variants of N. sylvestris origin showed less similarity to each other (80.5 % in the genomic region; 90.1 % in the coding sequence) than that between the NtTERT-C/s and NtTERT-C/t variants (93.6 and 97.2 %, respectively). The NtTERT-D variant was truncated at the 5' end, and indels indicated that it was a pseudogene. All tobacco variants were transcribed and alternatively spliced sequences were detected. Analysis of gene arrangements uncovered a novel exon in the N-terminal domain of TERT variants, a feature that is likely to be commonly found in Solanaceae species. In addition, species-specific duplications were observed within exon 5. The putative function, copy number and evolutionary origin of these NtTERT sequence variants are discussed.

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Mol Gen Genet. 1998 Dec;260(5):470-4 PubMed

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FEBS Lett. 1995 May 1;364(1):33-5 PubMed

Gene. 2007 Oct 1;400(1-2):16-24 PubMed

Mol Phylogenet Evol. 2010 Apr;55(1):99-112 PubMed

Biochimie. 2011 Nov;93(11):1916-21 PubMed

Genome Res. 2009 Jan;19(1):42-56 PubMed

FEBS Lett. 2000 Feb 11;467(2-3):305-10 PubMed

Mol Biol Evol. 2010 Apr;27(4):781-99 PubMed

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