Chromatin Assembly Factor 1 (CAF1) is a three-subunit H3/H4 histone chaperone responsible for replication-dependent nucleosome assembly. It is composed of CAC 1-3 in yeast; p155, p60, and p48 in humans; and FASCIATA1 (FAS1), FAS2, and MULTICOPY SUPPRESSOR OF IRA1 in Arabidopsis thaliana. We report that disruption of CAF1 function by fas mutations in Arabidopsis results in telomere shortening and loss of 45S rDNA, while other repetitive sequences (5S rDNA, centromeric 180-bp repeat, CACTA, and Athila) are unaffected. Substantial telomere shortening occurs immediately after the loss of functional CAF1 and slows down at telomeres shortened to median lengths around 1 to 1.5 kb. The 45S rDNA loss is progressive, leaving 10 to 15% of the original number of repeats in the 5th generation of mutants affecting CAF1, but the level of the 45S rRNA transcripts is not altered in these mutants. Increasing severity of the fas phenotype is accompanied by accumulation of anaphase bridges, reduced viability, and plant sterility. Our results show that appropriate replication-dependent chromatin assembly is specifically required for stable maintenance of telomeres and 45S rDNA.

Division of Functional Genomics and Proteomics Department of Experimental Biology Faculty of Science Masaryk University CZ 61137 Brno Czech Republic

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Histone Chaperone Deficiency in Arabidopsis Plants Triggers Adaptive Epigenetic Changes in Histone Variants and Modifications

In-section Click-iT detection and super-resolution CLEM analysis of nucleolar ultrastructure and replication in plants

Telomeres and Their Neighbors

Telomerase Interaction Partners-Insight from Plants

G2/M-checkpoint activation in fasciata1 rescues an aberrant S-phase checkpoint but causes genome instability

Expansion of rDNA and pericentromere satellite repeats in the genomes of bank voles Myodes glareolus exposed to environmental radionuclides

The rDNA Loci-Intersections of Replication, Transcription, and Repair Pathways

Large tandem duplications affect gene expression, 3D organization, and plant-pathogen response

G4 Structures in Control of Replication and Transcription of rRNA Genes

Variation of 45S rDNA intergenic spacers in Arabidopsis thaliana

Identification of Nucleolus-Associated Chromatin Domains Reveals a Role for the Nucleolus in 3D Organization of the A. thaliana Genome

Homology-dependent repair is involved in 45S rDNA loss in plant CAF-1 mutants

Telomere repeat binding proteins are functional components of Arabidopsis telomeres and interact with telomerase

A telomerase-independent component of telomere loss in chromatin assembly factor 1 mutants of Arabidopsis thaliana

Three TERT genes in Nicotiana tabacum