Amniotic fluid soluble Toll-like receptor 2 in pregnancies complicated by preterm prelabor rupture of membranes
Language English Country Great Britain, England Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Amniocentesis MeSH
- Amnion microbiology MeSH
- Chorioamnionitis diagnosis pathology MeSH
- Adult MeSH
- Gestational Age MeSH
- Cohort Studies MeSH
- Humans MeSH
- Infant, Newborn MeSH
- Amniotic Fluid chemistry microbiology MeSH
- Fetal Membranes, Premature Rupture metabolism microbiology MeSH
- Prospective Studies MeSH
- Reference Values MeSH
- Solubility MeSH
- Respiratory Distress Syndrome, Newborn diagnosis MeSH
- Pregnancy MeSH
- Toll-Like Receptor 2 analysis MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Infant, Newborn MeSH
- Pregnancy MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- TLR2 protein, human MeSH Browser
- Toll-Like Receptor 2 MeSH
OBJECTIVE: To determine amniotic fluid soluble Toll-like receptor 2 (sTLR2) levels in PPROM according to the presence of microbial invasion of the amniotic cavity (MIAC), histological chorioamnionitis (HCA), and both these conditions. To test the cutoff level of 222.7 ng/mL, as proposed in our previous study, in order to distinguish women with both MIAC and HCA. METHODS: 169 women with a gestational age between 24+0 and 36+6 weeks were included in a prospective cohort study. Amniocenteses were performed, and sTLR2 in the amniotic fluid were determined using ELISA. RESULTS: Women with MIAC had higher sTLR2 levels (median 113.2 ng/mL) than those without MIAC (median 47.1 ng/mL; p < 0.0001). Women with HCA did not have a higher sTLR2 level (median 52.6 ng/mL) compared with women without HCA (median 47.1 ng/mL; p = 0.23). Women with both MIAC and HCA had higher sTLR2 levels (median: 311.3 ng/mL) than other women (17.5 ng/mL; p < 0.0001). The cutoff level 222.7 ng/mL had a sensitivity of 63%, a specificity of 98%, and a likelihood ratio of 40.3 for the prediction of both MIAC and HCA. CONCLUSIONS: Amniotic fluid sTLR2 is a promising predictor of both MIAC and HCA with high specificity in PPROM.
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